Abstract
The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.
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Acknowledgements
We thank M. Asashima for encouraging this work, K. Rodolfa, A. Kweudjeu and C. Balatbat for excellent technical support, S. Sullivan, D. Huangfu and Q. Zhou for critically reading the manuscript. D.A.M. is an investigator of the Howard Hughes Medical Institute.
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K.O., L.C., M.B., C.A.C., K.R.C. and D.A.M. designed the experiments. K.O., L.C., M.B., R.J.M. and C.S.F. performed experiments. K.O., L.C., M.B., Y.S., C.A.C., K.R.C. and D.A.M. analyzed the data obtained. K.O. and D.A.M. drafted the manuscript. K.O., L.C., M.B., R.J.M., C.S.F., Y.S., C.A.C., K.R.C. and D.A.M. provided revisions to the manuscript.
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Osafune, K., Caron, L., Borowiak, M. et al. Marked differences in differentiation propensity among human embryonic stem cell lines. Nat Biotechnol 26, 313–315 (2008). https://doi.org/10.1038/nbt1383
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DOI: https://doi.org/10.1038/nbt1383
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