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Fusarium graminearum A 3/5 as a Novel Host for Heterologous Protein Production

Bio/Technology volume 13, pages 1479–1483 (1995)Cite this article

Abstract

We describe a novel fungal expression system which utilizes the Quorn® myco-protein fungus Fusarium graminearum A 3/5. A transformation system was developed for F. graminearum and was used to introduce the coding and regulatory regions of a trypsin gene from Fusarium oxysporum. The protein was efficiently expressed, processed and secreted by the recombinant host strain. In addition, the promoter and terminator of the F. oxysporum trypsin gene have been successfully utilized to drive the expression of a cellulase gene from Scytalidium thermophilum and a lipase gene from Thermomyces lanuginosus in F. graminearum.

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Author notes

  1. John C. Royer: Corresponding author.

Authors and Affiliations

  1. Novo Nordisk Biotech, Inc., 1445 Drew Avenue, Davis, California, 95616

    John C. Royer, Donna L. Moyer, Sarah G. Reiwitch, Mark S. Madden, Ejner Bech Jensen, Stephen H. Brown, Cynthia C. Yonker, James A. Johnstone, Elizabeth J. Golightly, Wendy T. Yoder & Jeffrey R. Shuster

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Royer, J., Moyer, D., Reiwitch, S. et al. Fusarium graminearum A 3/5 as a Novel Host for Heterologous Protein Production. Nat Biotechnol 13, 1479–1483 (1995). https://doi.org/10.1038/nbt1295-1479

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