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Getting the glycosylation right: Implications for the biotechnology industry

Nature Biotechnology volume 14, pages 975–981 (1996)Cite this article

Abstract

Glycosylation is the most extensive of all the posttranslational modifications, and has important functions in the secretion, antigenicity and clearance of glycoproteins. In recent years major advances have been made in the cloning of glycosyltransferase enzymes, in understanding the varied biological functions of carbohydrates, and in the accurate analysis of glycoprotein heterogeneity. In this review we discuss the impact of these advances on the choice of a recombinant host cell line, in optimizing cell culture processes, and in choosing the appropriate level of glycosylation analysis for each stage of product development.

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  1. Nigel Jenkins: e-mail: njenkins@dmu.ac.uk.

Authors and Affiliations

  1. Department of Biological Sciences, DeMontfort University, Leicester, LE1 9BH, UK

    Nigel Jenkins, Raj B. Parekh & David C. James

  2. Oxford Glycosciences, Hitching Court, Blacklands Way, Abingdon, OX14 1RG, UK

    Raj B. Parekh

  3. Research School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK

    David C. James

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Jenkins, N., Parekh, R. & James, D. Getting the glycosylation right: Implications for the biotechnology industry. Nat Biotechnol 14, 975–981 (1996). https://doi.org/10.1038/nbt0896-975

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