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Charging two for the price of one

Nature Structural Biology volume 8, pages 189–191 (2001)Cite this article

A structure of the complex of tRNAGlu and class I glutamyl-tRNA synthetase from Thermus thermophilus provides new details of tRNA recognition, and an accompanying mutational study provides a model for how a discriminating enzyme could have evolved from a less discriminating predecessor.

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Figure 1: Formation of Glu-tRNAGlu and Gln-tRNAGln by discriminating and nondiscriminating GluRS.
Figure 2: Model for the evolution of the GlxRS family.

References

  1. Ibba, M. & Soll, D. Science 286, 1893–1897 (1999).

    Article  CAS  Google Scholar 

  2. Ibba, M., Becker, H.D., Stathopoulos, C., Tumbula, D.L. & Soll, D. Trends Biochem. Sci. 25, 311–316 (2000).

    Article  CAS  Google Scholar 

  3. Wilcox, M. & Nirenberg, M. Proc. Natl. Acad. Sci. USA 61, 229–236 (1968).

    Article  CAS  Google Scholar 

  4. Bult, C.J. et al. Science 273, 1058–1073 (1996).

    Article  CAS  Google Scholar 

  5. Smith, D.R. et al. J. Bacteriol. 179, 7135–7155 (1997).

    Article  CAS  PubMed Central  Google Scholar 

  6. Ibba, M. & Söll, D. Ann. Rev. Biochem. 69, 617–650 (2000).

    Article  CAS  Google Scholar 

  7. Sekine, S., Nureki, O., Shimada, A., Vassylyev, D.G. & Yokoyama, S. Nature Struct. Biol. 203–206 (2001).

  8. Carter, C.W., Jr. Annu. Rev. Biochem. 62, 715–748 (1993).

    Article  CAS  Google Scholar 

  9. Nureki, O. et al. Science 267, 1958–1965 (1995).

    Article  CAS  Google Scholar 

  10. Rould, M.A., Perona, J.J., Soll, D. & Steitz, T.A. Science 246, 1135–1142 (1989).

    Article  CAS  Google Scholar 

  11. Weygand-Durasevic, I., Schwob, E. & Söll, D. Proc. Natl. Acad. Sci. USA 90, 2010–2014 (1993).

    Article  CAS  Google Scholar 

  12. Delarue, M. & Moras, D. BioEssays 15, 1–13 (1993).

    Article  Google Scholar 

  13. Giege, R., Sissler, M. & Florentz, C. Nucleic Acids Res. 26, 5017–5035 (1998).

    Article  CAS  PubMed Central  Google Scholar 

  14. Rould, M.A., Perona, J.J. & Steitz, T.A. Nature 352, 213–218 (1991).

    Article  CAS  Google Scholar 

  15. Shi, H. & Moore, P.B. RNA 6, 1091–1105 (2000).

    Article  CAS  PubMed Central  Google Scholar 

  16. Sylvers, L.A., Rogers, K.C., Shimizu, M., Ohtsuka, E. & Soll, D. Biochemistry 32, 3836–3841 (1993).

    Article  CAS  Google Scholar 

  17. Auld, D.S. & Schimmel, P. Science 267, 1994–1996 (1995).

    Article  CAS  PubMed Central  Google Scholar 

  18. Woese, C.R., Olsen, G.J., Ibba, M. & Soll, D. Microbiol. Mol. Biol. Rev. 64, 202–236 (2000).

    Article  CAS  PubMed Central  Google Scholar 

  19. Doolittle, R.F. & Handy, J. Curr. Opin. Genet. Dev. 8, 630–636 (1998).

    Article  CAS  Google Scholar 

  20. Wolf, Y.I., Aravind, L., Grishin, N.V. & Koonin, E.V. Genome Res. 9, 689–710 (1999).

    CAS  PubMed  Google Scholar 

  21. Siatecka, M., Rozek, M., Barciszewski, J. & Mirande, M. Eur. J. Biochem. 256, 80–87 (1998).

    Article  CAS  Google Scholar 

Download references

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  1. the Department of Biochemistry, University of Vermont College of Medicine, Burlington, 05405, Vermont, USA

    Christopher S. Francklyn

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Francklyn, C. Charging two for the price of one. Nat Struct Mol Biol 8, 189–191 (2001). https://doi.org/10.1038/84901

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