Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Involvement of nucleotide-excision repair in msh2 pms1-independent mismatch repair

Nature Genetics volume 21, pages 314–317 (1999)Cite this article

Abstract

Nucleotide-excision repair (NER) and mismatch repair (MMR) are prominent examples of highly conserved DNA repair systems which recognize and replace damaged and/or mispaired nucleotides in DNA. In humans, inheritable defects in components of the NER system are associated with severe diseases such as xeroderma pigmentosum (XP) and Cockayne syndrome1 (CS), whereas inactivation of MMR is accompanied by predisposition to certain types of cancer2. In Schizosaccharomyces pombe, the msh2- and pms1-dependent long-patch MMR system efficiently corrects small insertion/deletion loops and all base-base mismatches, except C/C. Up to 70% of C/C mismatches generated in recombination intermediates, and to a lesser extent also other base-base mismatches, are thought to undergo correction by a minor, short-patch excision repair system3,4. We identify here the NER genes rhp14, swi10 and rad16 as components of this repair pathway and show that they act independently of msh2 and pms1.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Structure of ade6, mutations used in this study and formation of mismatches.

Similar content being viewed by others

Accession codes

Accessions

GenBank/EMBL/DDBJ

References

  1. Sancar, A. DNA excision repair. Annu. Rev. Biochem. 65, 43–81 (1996).

    Article  CAS  Google Scholar 

  2. Modrich, P. & Lahue, R. Mismatch repair in replication fidelity, genetic recombination, and cancer biology. Annu. Rev. Biochem. 65, 101–133 ( 1996).

    Article  CAS  Google Scholar 

  3. Schär, P., Munz, P. & Kohli, J. Meiotic mismatch repair quantified on the basis of segregation patterns in Schizosaccharomyces pombe. Genetics 133, 815–824 (1993).

    Google Scholar 

  4. Schär, P. & Kohli, J. Marker effects of G to C transversions on intragenic recombination and mismatch repair in Schizosaccharomyces pombe. Genetics 133, 825– 835 (1993).

    Google Scholar 

  5. Rudolph, C. et al. The msh2 gene of Schizosaccharomyces pombe is involved in mismatch repair, mating-type switching, and meiotic chromosome organization. Mol. Cell. Biol. 19, 241– 250 (1999).

    Article  CAS  Google Scholar 

  6. Schär, P., Baur, M., Schneider, C. & Kohli, J. Mismatch repair in Schizosaccharomyces pombe requires the mutL homologous gene pms1: molecular cloning and functional analysis. Genetics 146, 1275–1286 (1997).

    Google Scholar 

  7. Aboussekhra, A. & Wood, R.D. Repair of UV-damaged DNA by mammalian cells and Saccharomyces cerevisiae. Curr. Opin. Genet. Dev. 4, 212–220 (1994).

    Article  CAS  Google Scholar 

  8. Lehmann, A.R. Molecular biology of DNA repair in the fission yeast Schizosaccharomyces pombe. Mutat. Res. 363, 147– 161 (1996).

    Article  Google Scholar 

  9. Rödel, C., Kirchhoff, S. & Schmidt, H. The protein sequence and some intron positions are conserved between the switching gene swi10 of Schizosaccharomyces pombe and the human excision repair gene ERCC1. Nucleic Acids Res. 20, 6347–6353 ( 1992).

    Article  Google Scholar 

  10. Carr, A.M. et al. The rad16 gene of Schizosaccharomyces pombe: a homolog of the RAD1 gene of Saccharomyces cerevisiae. Mol. Cell. Biol. 14, 2029–2040 (1994).

    Article  CAS  Google Scholar 

  11. Schär, P. & Kohli, J. Preferential strand transfer and hybrid DNA formation at the recombination hotspot ade6-M26 of Schizosaccharomyces pombe. EMBO J. 13 , 5212–5219 (1994).

    Article  Google Scholar 

  12. Schär, P. & Jiricny, J. in Nucleic Acids and Molecular Biology: Eukaryotic Mismatch Repair (eds Eckstein, F. & Lilley, D.M.J.) 199–247 (Springer-Verlag Berlin, Heidelberg, 1998).

    Google Scholar 

  13. Montelone, B.A., Gilbertson, R., Nassar, R., Giroux, C. & Malone, R.E. Analysis of the spectrum of mutations induced by the rad3-102 mutator allele of yeast. Mutat. Res. 267, 55–66 ( 1992).

    Article  CAS  Google Scholar 

  14. Montelone, B.A. & Malone, R.E. Analysis of the rad3-101 and rad3-102 mutations of Saccharomyces cerevisiae: implications for structure/function of Rad3 protein. Yeast 10, 13–27 ( 1994).

    Article  CAS  Google Scholar 

  15. Kirkpatrick, D.T. & Petes, T.D. Repair of DNA loops involves DNA-mismatch and nucleotide-excision repair proteins. Nature 387, 929–931 ( 1997).

    Article  CAS  Google Scholar 

  16. Sekelsky, J.J., McKim, K.S., Chin, G.M. & Hawley, R.S. The Drosophila meiotic recombination gene mei-9 encodes a homologue of the yeast excision repair protein Rad1. Genetics 141, 619– 627 (1995).

    CAS  Google Scholar 

  17. Carpenter, A.T.C. Mismatch repair, gene conversion, and crossing-over in two recombination-defective mutants of Drosophila. Proc. Natl Acad. Sci. USA 79 , 5961–5965 (1982).

    Article  CAS  Google Scholar 

  18. Huang, J.-C., Hsu, D.S., Kazantsev, A. & Sancar, A. Substrate spectrum of human excinuclease: repair of abasic sites, methylated bases, mismatches, and bulky adducts. Proc. Natl Acad. Sci. USA 91, 12213–12217 (1994).

    Article  CAS  Google Scholar 

  19. Gutz, H., Heslot, H., Leupold, U. & Loprieno, N. in Handbook of Genetics, Schizosaccharomyces pombe (ed. King, R.C.) 395– 446 (Plenum Press, New York, 1994).

    Google Scholar 

  20. Szankasi, P., Heyer, W.-D., Schuchert, P. & Kohli, J. DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe. Wild-type and mutant alleles including the recombination hot spot allele ade6-M26. J. Mol. Biol. 204, 917– 925 (1988).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the Swiss National Science Foundation and a fellowship of the Deutsche Forschungsgemeinschaft to O.F.

Author information

Authors and Affiliations

  1. Institute of General Microbiology, University of Bern , Baltzer-Strasse 4, Bern, CH-3012, Switzerland

    Oliver Fleck, Elisabeth Lehmann & Jürg Kohli

  2. Institute for Medical Radiobiology, August-Forel Strasse 7, Zürich, CH-8008, Switzerland

    Primo Schär

Authors
  1. Oliver Fleck
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elisabeth Lehmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Primo Schär
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jürg Kohli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Oliver Fleck.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fleck, O., Lehmann, E., Schär, P. et al. Involvement of nucleotide-excision repair in msh2 pms1-independent mismatch repair. Nat Genet 21, 314–317 (1999). https://doi.org/10.1038/6838

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/6838

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing