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Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli

Nature volume 351, pages 371–377 (1991)Cite this article

Abstract

Examination of the structure of Escherichia coli heat-labile enterotoxin in the AB5 complex at a resolution of 2.3 Å reveals that the doughnut-shaped B pentamer binds the enzymatic A subunit using a hairpin of the A2 fragment, through a highly charged central pore. Putative ganglioside GM1-binding sites on the B subunits are more than 20 Å removed from the membrane-crossing Al subunit. This ADP-ribosylating (Al) fragment of the toxin has structural homology with the catalytic region of exotoxin A and hence also to diphtheria toxin.

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Authors and Affiliations

  1. BIOSON Research Institute, University of Groningen, Nijenborgh 16, 9747 AG, Groningen, The Netherlands

    Titia K. Sixma, Sylvia E. Pronk, Kor H. Kalk, Ellen S. Wartna, Ben A. M. van Zanten, Bernard Witholt & Wim G. J. Hoi

  2. Laboratory of Biochemistry, Department of Chemistry, University of Groningen, Nijenborgh 16, 9747 AG, Groningen, The Netherlands

    Bernard Witholt

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  1. Titia K. Sixma
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Sixma, T., Pronk, S., Kalk, K. et al. Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli. Nature 351, 371–377 (1991). https://doi.org/10.1038/351371a0

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