Abstract
IN the Caenorhabditis elegans hermaphrodite germ line, sperm and then oocytes are made from a common pool of germ-cell precursors. The decision to differentiate as a sperm or an oocyte is regulated by the sex-determining gene,fem-3. Expression of fem-3 in the hermaphrodite germ line directs spermatogenesis and must be negatively regulated to allow the switch to oogenesis1,2. In adult hermaphrodites (which are producing oocytes), mostfem-3 RNA is found in the germ line3, consistent with both the requirement for fem-3 in hermaphrodite spermatogenesis and the maternal effects of fem-3 on embryonic sex determination1,2Whereas loss-of-function mutants in fem-3 produce only oocytes, hermaphrodites carrying any of nine fem-3 gain-of-function (gf) mutations make none; instead sperm are produced continuously and in vast excess over wild-type amounts1Genetic analyses suggest that fem-3(gf) mutations have escaped a negative control required for the switch to oogenesis1. Here we report that all nine fem-3(gf) mutants carry sequence alterations in the fem-3 3′ untranslated region (3′ UTR). There is no increase in the steady-state level of fem-3(gf) RNA over wild-type, but there is an increase in the polyadenylation of fem-3(gf) RNA that is coincident with the unregulated fem-3 sactivity. Results of a titration experiment support the hypothesis that a regulatory factor may bind the fem-3 3′ UTR. We speculate that fem-3 RNA is regulated through its 3′ UTR by binding a factor that inhibits translation, and discuss the idea that this control may be part of a more general regulation of maternal RNAs.
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Ahringer, J., Kimble, J. Control of the sperm–oocyte switch in Caenorhabditis elegans hermaphrodites by the fem-3 3′ untranslated region. Nature 349, 346–348 (1991). https://doi.org/10.1038/349346a0
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DOI: https://doi.org/10.1038/349346a0
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