Abstract
THE role of microfilaments in secretion has been much debated1–4. If secretion involves a sliding filament mechanism similar to muscle contraction5, microfilaments must attach at least transiently to vesicle membranes. Indeed, there is evidence that contractile proteins are associated with secretory vesicles (chromaffin granules) of adrenal medulla6,7. Moreover, the outer (cytoplasmic) surface of secretory vesicles must provide ‘anchoring molecules’ for microfilaments. In skeletal muscle, the structural protein α-actinin located in the Z-line8 serves as such an anchoring molecule. An α-actinin-like protein was isolated from a variety of non-muscle cells9,10 and there is evidence that the same protein is attached to the inner (cytoplasmic) surface of the plasma membrane of non-muscle cells in association with microfilaments11–14. Using a monospecific antibody to porcine α-actinin (Fig. 1) we have demonstrated the presence of this protein in secretory vesicle membranes from chromaffin cells of the adrenal medulla and from platelets.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Allison, A. C. in Locomotion of Tissue Cells. Ciba Fdn Symp 14 (ed. Porter R. & Fitzsimons. D. W.) 109–143 (Associated Scientific, Amsterdam, 1973).
Cooke, P. & Poisner, A. M. Cytobiology 13, 442–450 (1976).
Orci, L., Gabbay, K. H. & Malaisse, W. J. Science 175, 1128–1130 (1972).
Orr, T. S. C., Hall, D. & Allison, A. C. Nature 236, 350–351 (1972).
Huxley, H. E. Science 164, 1356–1366 (1969).
Burridge, K. & Phillips, J. H. Nature 254, 526–529 (1975).
Gabbiani, G., Da Prada, M., Richards, G. & Pletscher, A. Proc. Soc. exp. Biol. Med. 152, 135–138 (1976).
Masaki, T., Endo, M. & Ebashi, S. J. Biochem. 62, 630–632 (1967).
Schollmeyer, J. V. et al. J. Cell Biol. 63, 304a (1974).
Lazarides, E. & Burridge, K. Cell 6, 289–298 (1975).
Mooseker, M. S. in Cold Spring Harbor Conferences on Cell Proliferation. 3 B, 631–650 (Cold Spring Harbor Labs, 1976).
Schollmeyer, J. L., Furcht, L. T., Goll, D. E., Robson, R. M. & Stromer, M. H. in Cold Spring Harbor Conferences on Cell Proliferation, 3 A, 361–387 (Cold Spring Harbor Labs., 1976).
Lazarides, E. J. Cell Biol. 68, 202–219 (1976).
Franke, W. W. et al. J. microse. Biol. Cell (in the press).
Goll, D. E., Suzuki, A., Temple, J. & Holmes, G. R. J. molec. Biol. 67, 469–488 (1972).
Cuatrecasas, P., Wilcheck, M. & Anderson, C. B. Proc. natn. Acad. Sci. U.S.A. 61, 636–643 (1968).
Fuller, G. M., Brinkley, B. R. & Boughter, J. M. Science 187, 948–950 (1975).
Lamelin, J. P., Lisowska-Bernstein, B., Matter, A., Ryser, J. E. & Vassalli, P. J. exp. Med. 136, 984–1007 (1972).
Chaponnier, C., Kohler, L. & Gabbiani, G. Clin. exp. Immunol. 27, 278–284 (1977).
DaPrada, M., v. Berlepsch, K. & Pletscher, A. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 275, 315–322 (1972).
Mustard, J. F. & Packham, M. A. Pharmac. Rev. 22, 97–187 (1970).
Laemmli, U. K. Nature 227, 680–685 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
JOCKUSCH, B., BURGER, M., PRADA, M. et al. α-Actinin attached to membranes of secretory vesicles. Nature 270, 628–629 (1977). https://doi.org/10.1038/270628a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/270628a0
This article is cited by
-
Interaction of the cytoskeleton with the plasma membrane
The Journal of Membrane Biology (1987)
-
Diacylglycerol in large α-actinin/actin complexes and in the cytoskeleton of activated platelets
Nature (1985)
-
Chromaffin granule membrane–F-actin interactions are calcium sensitive
Nature (1982)
-
Immunohistochemical and immunocytochemical localization of myosin, chromogranin A and dopamine-?-hydroxylase in nerve cells in culture and in adrenal glands
Journal of Neurocytology (1980)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.