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Partial denaturation mapping of cloned histone DNA from the sea urchin Psammechinus miliaris

Nature volume 264, pages 31–34 (1976)Cite this article

Abstract

A cloned 5.6-kb DNA repeat unit 0f Psammechinus miliaris containing all five histone coding sequences of known order and polarity has been shown to fall clearly into a low melting and a high melting half by thermal denaturation. The topologies of the DNA sequences thus defined were determined by partial denaturation mapping in the electron microscope to gain further insight into the distribution of spacers and genes within histone DNA.

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Authors and Affiliations

  1. Institut für Molekularbiologie II der Universität Zürich, Winterthurerstrasse 266A, 8057, Zürich, Switzerland

    Rudolf Portmann, Walter Schaffner & Max Birnstiel

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  1. Rudolf Portmann
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  2. Walter Schaffner
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  3. Max Birnstiel
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Portmann, R., Schaffner, W. & Birnstiel, M. Partial denaturation mapping of cloned histone DNA from the sea urchin Psammechinus miliaris. Nature 264, 31–34 (1976). https://doi.org/10.1038/264031a0

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  • DOI: https://doi.org/10.1038/264031a0

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