Abstract
DENSITY-GRADIENT centrifugation of both the velocity and equilibrium types has been applied for separating and purifying plant viruses such as tobacco mosaic and potato yellows1, and also very recently to animal viruses such as Rous sarcoma2, Shope fibroma3 and polyoma4. Equilibrium banding with satisfactory recovery of biological activity has usually been achieved in rubidium or cæsium chlorides after centrifugation for approximately 24 hr. Some viruses are, however, inactivated by these salts, and others, as, for example, Rous sarcoma, require stabilization by albumin and citrate2. Non-equilibrium sedimentation in glycerol gradients has been used to purify Shope papilloma3 ; and reasonably sharp peaks of influenza and Newcastle disease viruses, and also of vaccinia hæmagglutinin, may be obtained after brief centrifugation in sucrose gradients of the velocity-sedimentation type5.
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References
Brakke, M. K., Virol., 6, 96 (1958).
Crawford, L. V., Virol., 12, 143 (1960).
Williams, R. C., Kass, S. J., and Knight, C. A., Virol., 12, 48 (1960).
Smith, J. D., Freeman, G., Vogt, M., and Dulbecco, R., Virol., 12, 185 (1960).
McCrea, J. F., and O'Loughlin, J. (unpublished results, 1959).
McCrea, J. F., and Harris, R. (in preparation).
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McCREA, J., EPSTEIN, R. & BARRY, W. Use of Potassium Tartrate for Equilibrium Density-gradient Centrifugation of Animal Viruses. Nature 189, 220–221 (1961). https://doi.org/10.1038/189220a0
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DOI: https://doi.org/10.1038/189220a0
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