Abstract
Centrosome duplication is a key requirement for bipolar spindle formation and correct segregation of chromosomes during cell division. In a manner highly reminiscent of DNA replication, the centrosome must be duplicated once, and only once, in each cell cycle. How centrosome duplication is regulated and coordinated with other cell-cycle functions remains poorly understood. Here, we have established a centrosome duplication assay using mammalian somatic cells. We show that centrosome duplication requires the activation of E2F transcription factors and Cdk2–cyclin A activity.
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Acknowledgements
We thank Y.-D. Stierhof for the electron microscopic data in Fig. 1, N. Roggli for help with artwork, and several colleagues for kind gifts of plasmids, particularly E. Harlow for Cdk1, 2 and 3, J. Massagué for p27Kip1, S. Reed for cyclin E , J. Pines for cyclin A , C.-L. Wu for DP1, and K. Helin for E2F. We also thank B. Amati as well as all members of the laboratory for stimulating discussions. This work was supported by grants from the Swiss National Science Foundation, the Swiss Cancer League, the Human Frontier Science Program and the Canton of Geneva.
Correspondence and requests for materials should be addressed to E.A.N.
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Meraldi, P., Lukas, J., Fry, A. et al. Centrosome duplication in mammalian somatic cells requires E2F and Cdk2–Cyclin A. Nat Cell Biol 1, 88–93 (1999). https://doi.org/10.1038/10054
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DOI: https://doi.org/10.1038/10054
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