Abstract
MOST antigens must be processed intracellularly before they can be presented, in association with major histocompatibility complex (MHC) molecules at the cell surface, for recognition by the antigen-specific receptor of T cells1–3. This processing appears to involve cleavage of protein antigens to Smaller peptides4–7. Only certain fragments of any protein can serve as T-cell epitopes and this is, at least in part, determined by the requirement that peptides be able to bind the MHC molecules8–10. Class I restricted antigens are derived from proteins, such as viral antigens, that are synthesized within the presenting cell11–13.Many of these antigens are cytosolic proteins and recent evidence suggests that it is in the cytosol that these proteins are processed to produce either the antigenic peptides or processed intermediates13–16. How and where these processed cytosolic antigens cross the membrane of the vacuolar system and bind to the extracellular domain of the class I molecule is not known but one obvious site for this process is the endoplasmic reticulum (ER), because this organelle is specialized to translocate proteins across the membrane from the cytosol into the secretory system. Based on this model, we reasoned that if we could pharmacologically block the movement of proteins out of the ER, endogenous antigen presentation would cease. An agent which causes such an effect is available17,18 —the fungal antibiotic Brefeldin A (BFA). Consistent with the above hypothesis, we report that BFA completely abolishes the ability of a cell to present endogenously synthesized antigens to class I restricted cytotoxic T cells.
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References
Unanue, E. R. A. Rev. Immun. 2, 395 (1984).
Morrison, L. A., Lukacher, A. E., Braciale, V. L., Fan, D. P. & Braciale, T. J. J. exp. Med. 163, 903–921 (1986).
Yewdell, J. W., Bennink, J. R. & Hosaka, Y. Science 239, 637–640 (1988).
Shimonkevitz, R., Colon, S., Kappler, J. W. & Grey, H. M. J. Immun. 133, 2067–2074 (1984).
Maryanski, J. L., Pala, P., Corrudin, G., Jordan, B. R. & Cerrotini, J. C. Nature 324, 578–579 (1986).
Townsend, A. R. M. et al. Cell 44, 959–968 (1986).
Gotch, F., Rothbard, J., Howland, K., Townsend, A. & McMichael, A. Nature 326, 881–882 (1987).
Babbitt, B. P., Allen, P. M., Matsueda, G., Haber, E. & Unanue, E. R. Nature 317, 359–361 (1985).
Guillet, J.-G., Lai, M.-Z., Briner, T. J., Smith, J. A. & Gefter, M. L. Nature 324, 260–262 (1986).
Buus, S., Sette, A., Colon, S., Jenis, D. M. & Grey, H. M. Cell 47, 1071–1077 (1986).
Townsend, A. R. M., McMichael, A. J., Carter, N. P., Huddleston, J. A. & Brownlee, G. G. Cell 39, 13–25 (1984).
Gotch, F., McMichael, A., Smith, G. & Moss, B. J. exp. Med. 165, 408–416 (1987).
Townsend, A. R. M., Gotch, F. M. & Davey, J. Cell 42, 457–467 (1985).
Townsend, A. R. M., Bastin, J., Gould, K. & Brownlee, G. G. Nature 324, 575–577 (1986).
Townsend, A. et al. J. exp. Med. 168, 1211–1224 (1988).
Moore, M. W., Carbone, F. R. & Bevan, M. J. Cell 54, 777–785 (1988).
Takatsuki, A. & Tamura, G. Agric. Biol. Chem. 49, 899–902 (1985).
Misumi, Y. et al. J. Biol. Chem. 261, 11398–11403 (1986).
Lippincott-Schwartz, J., Yuan, L. C., Bonifacino, J. S. & Klausner, R. D. Cell 56, 801–813 (1989).
Kornfeld, R. & Kornfeld, S. A. Rev. Biochem. 54, 631–664 (1985).
Hogan, K. T. et al. J. exp. Med. 168, 725–736 (1988).
Palese, P., Tobita, K., Ueda, M., & Compans, R. W. Virology 61, 397–410 (1974).
Matlin, K. & Simones, K. Cell 34, 233–243 (1983).
Owen, M. J., Kissonerghis, A.-M., & Lodish, H. F. J. biol. Chem. 255, 9678–9684 (1980).
Samelson, L. E., Germain, R. N. & Schwartz, R. H. Proc. natn. Acad. Sci. U.S.A. 80, 6972–6976 (1983).
Ashwell, J. D., Cunningham, R. E., Noguchi, P. D. & Hernandez, D. J. exp. Med. 165, 173–194 (1987).
Bonifacino, J. S., Suzuki, C. K., Lippincott-Schwartz, J., Weissman, A. M. & Klausner, R. D. J. Cell Biol. (in the press).
Walter, P. & Lingappa, V. R. A. Rev. Cell Biol. 2, 499–516 (1986).
Lippincott-Schwartz, J., Bonifacino, J. S., Yuan, L. C. & Klausner, R. D. Cell 54, 209–220 (1988).
Chen, C., Bonifacino, J. S., Yuan, L. C. & Klausner, R. D. J. Cell Biol. 107, 2149–2161 (1988).
Cowan, E. P., Jelachich, M. L., Coligan, J. E. & Biddison, W. E. Proc. natn. Acad. Sci. U.S.A. 84, 5014–5018 (1987).
Samelson, L. E., Harford, J. B. & Klausner, R. D. Cell 43, 223–231 (1985).
Sussman, J. J. et al. Cell 52, 85–95 (1988).
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Nuchtern, J., Bonifacino, J., Biddison, W. et al. Brefeldin A implicates egress from endoplasmic reticulum in class I restricted antigen presentation. Nature 339, 223–226 (1989). https://doi.org/10.1038/339223a0
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DOI: https://doi.org/10.1038/339223a0
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