Abstract
Inflammatory bowel disease (IBD) is caused by an uncontrolled immune response in the intestinal lumen, leading to inflammation in genetically predisposed individuals. Immunotherapy may be a promising approach to the treatment of IBD. Here, we show that transforming growth factor-β1 (TGF-β1) gene-modified immature dendritic cells (imDCs) could enhance the inhibitory function of imDCs and delay the progress of IBD induced by dextran sodium sulfate in mice. The results of fluorescence-activated cell sorter (FACS) demonstrated that this protective effect is mediated partially by inducing CD4+Foxp3+ regulatory T cells (Tregs) in mesentery lymph nodes to control inflammation. In vitro experiments also supported this hypothesis. In conclusion, we provide evidence that TGF-β1-modified bone marrow-derived imDCs may have a therapeutic effect to IBD.
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Acknowledgements
We thank Professor Lin-Rong Lu for his critical review of the manuscript. This work was supported by the National Key Basic Research Program of China (Grant 2007CB512400), the National High Technology Research and Development Program of China (Grants 2006AA02A239 and 2007AA021102), the National Natural Science Foundation of China (Grant 30671909 and 30972725) and Natural Science Foundation of Zhejiang Province (Z2090042).
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Cai, Z., Zhang, W., Li, M. et al. TGF-β1 gene-modified, immature dendritic cells delay the development of inflammatory bowel disease by inducing CD4+Foxp3+ regulatory T cells. Cell Mol Immunol 7, 35–43 (2010). https://doi.org/10.1038/cmi.2009.107
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DOI: https://doi.org/10.1038/cmi.2009.107
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