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Allografting

Chimerism monitoring following allogeneic hematopoietic stem cell transplantation

Bone Marrow Transplantation volume 39, pages 529–535 (2007)Cite this article

Abstract

Information regarding the chimeric status of hematopoietic stem cell transplantation (HSCT) recipients is of great significance when comparing different conditioning and prophylactic therapies. In recent years, short tandem repeats/variable number tandem repeats (STRs/VNTRs) have emerged as the best tool for chimerism monitoring. However, the polymorphisms of STR/VNTR markers vary within and between ethnic groups. The issue is further complicated in a heterogeneous population such as occurs in the Indian subcontinent. In the present study, we attempted to devise a robust scheme to identify a set of polymorphic STRs/VNTRs most suitable for chimerism evaluation in north Indian HCST recipients. At first, we did genotyping of 11 STR and one VNTR in 1000 randomly chosen north Indian individuals to quantify different diversity parameters. Resulting data indicated that ApoB3′HVR, FES, VWA, D3S1358 and D16S310 were most polymorphic loci with the average heterozygosity being 0.756±0.17. Furthermore, all markers were genotyped in 77 HLA-matched donor–recipient pairs to evaluate the informativeness in differentiating donor's and recipient's cells. A panel of seven markers (ApoB3HVR-D3S1358-HUM-THO1-VWF-1-D16S310-FES-VWA) differentiated 98.70% of donor–recipient pairs. This set of markers also successfully monitored the graft status in 14 HSCT cases during multiple time points following HSCT. The results were compared to the commercially available AmpF/STR SGM Plus multiplex PCR kit (Applied Biosystems, Foster City, CA, USA). Our findings established that the panel of seven markers we identified was more cost-effective and informative.

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Acknowledgements

We thank Indian Council of Medical Research (ICMR) New Delhi and the Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow for providing various lab facilities and for other assistance. We appreciate the assistance of Dr Catherine Ivory in carrying out the linguistic modifications of the manuscript.

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Authors and Affiliations

  1. Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical School, Lucknow, Uttar Pradesh, India

    S Talwar, F Khan & S Agrawal

  2. Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada,

    F Khan

  3. Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical School, Lucknow, Uttar Pradesh, India

    S Nityanand

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  1. S Talwar
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  2. F Khan
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  3. S Nityanand
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  4. S Agrawal
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Correspondence to S Agrawal.

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Talwar, S., Khan, F., Nityanand, S. et al. Chimerism monitoring following allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant 39, 529–535 (2007). https://doi.org/10.1038/sj.bmt.1705626

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