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Stem Cell Mobilisation and Usage

Mobilized peripheral blood stem cells provide rapid reconstitution but impaired long-term engraftment in a mouse model

Bone Marrow Transplantation volume 39, pages 401–409 (2007)Cite this article

Abstract

In this study, we use competitive repopulation to compare the quality and frequency of stem cells isolated from mobilized blood with stem cells isolated from bone marrow (BM) in a mouse model. Lin−Sca-1+c-Kit+ (LSK) cells were harvested from control BM and peripheral blood of mice following granulocyte colony-stimulating factor (G-CSF) administration. LSK cells were used because of their resemblance to human CD34+ cells. We confirmed that transplantation of phenotypically defined mobilized peripheral blood (MPB) stem cells results in rapid recovery of blood counts. However, in vitro results indicated that LSK cells purified from MPB had lower cobblestone area-forming cell day 35 activity compared to BM. Additionally, evaluation of chimerism after co-transplantation of LSK cells purified from blood and BM revealed that MPB stem cells contained 25-fold less repopulation potential compared to BM stem cells. Competitive repopulating unit frequency analysis showed that freshly isolated MPB LSK cells have 8.8-fold fewer cells with long-term repopulating ability compared to BM LSK cells. Secondary transplantation showed no further decline in contribution of hematopoiesis relative to BM. We conclude that the reduced frequency of stem cells within the LSK population of MPB, rather than poorer quality, causes the reduced repopulation potential.

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Acknowledgements

We thank Geert Mesander and Henk Moes for their assistance with cell sorting and the staff of our animal facility for taking care of the mice. No conflicts of interest exists between authors. This work was supported by Grants from the: European Union (EU-LSHC-CT-2004–503436) and the Ubbo Emmius Foundation.

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Authors and Affiliations

  1. Department of Cell Biology, Section Stem Cell Biology, University Medical Centre Groningen, Groningen, The Netherlands

    J S G Yeoh, A Ausema, P Wierenga, G de Haan & R van Os

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  1. J S G Yeoh
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  2. A Ausema
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  3. P Wierenga
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  4. G de Haan
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  5. R van Os
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Correspondence to R van Os.

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Yeoh, J., Ausema, A., Wierenga, P. et al. Mobilized peripheral blood stem cells provide rapid reconstitution but impaired long-term engraftment in a mouse model. Bone Marrow Transplant 39, 401–409 (2007). https://doi.org/10.1038/sj.bmt.1705601

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