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Stem Cells and Tissue Regeneration

When is a stem cell really a stem cell?

Summary:

While bone marrow transplantation has long been established as an effective approach to the clinical management of a variety of malignant and nonmalignant diseases, the future application of pluripotent stem cells in transplant settings promises to deliver this therapy to a much broader range of indications. In this review, I summarize the emerging field of embryonic stem cell biology in the context of potential clinical applications and regulatory issues.

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References

  1. Ford CE, Hamerton JL, Barnes DWH et al. Cytological identification of radiation-chimeras. Nature 1956; 177: 452–454.

    Article  CAS  Google Scholar 

  2. Wright DE, Wagers AJ, Gulati AP et al. Physiological migration of hematopoietic stem and progenitor cells. Science 2001; 294: 1933–1936.

    Article  CAS  Google Scholar 

  3. Till JE, McCulloch EA . A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res 1961; 14: 213–222.

    Article  CAS  Google Scholar 

  4. Bradley TR, Metcalf D . The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci 1966; 44: 287–299.

    Article  CAS  Google Scholar 

  5. Thean LE, Hodgson GS, Bertoncello I et al. Characterization of megakaryocyte spleen colony-forming units by response to 5-fluorouracil and by unit gravity sedimentation. Blood 1983; 62: 896–901.

    CAS  PubMed  Google Scholar 

  6. Visser JWM, Bauman JGJ, Mulder AH et al. Isolation of murine pluripotent hemopoietic stem cells. J Exp Med 1984; 159: 1576–1590.

    Article  CAS  Google Scholar 

  7. Spangrude GJ, Heimfeld S, Weissman IL . Purification and characterization of mouse hematopoietic stem cells. Science 1988; 241: 58–62.

    Article  CAS  Google Scholar 

  8. Orlic D, Bodine DM . What defines a pluripotent hematopoietic stem cell (PHSC): will the real PHSC please stand up! Blood 1994; 84: 3991–3994.

    CAS  PubMed  Google Scholar 

  9. Korbling M, Katz RL, Khanna A et al. Hepatocytes and epithelial cells of donor origin in recipients of peripheral-blood stem cells. N Engl J Med 2002; 346: 738–746.

    Article  Google Scholar 

  10. Abkowitz JL . Can human hematopoietic stem cells become skin, gut, or liver cells? N Engl J Med 2002; 346: 770–772.

    Article  Google Scholar 

  11. Lewis R . A stem cell legacy: Leroy Stevens. Scientist 2000; 14: 19.

    CAS  Google Scholar 

  12. Stevens LC . Studies on transplantable testicular teratomas of strain 129 mice. J Natl Cancer Inst 1958; 20: 1257–1270.

    Article  CAS  Google Scholar 

  13. Mintz B, Illmensee K . Normal genetically mosaic mice produced from malignant teratocarcinoma cells. Proc Natl Acad Sci USA 1975; 72: 3585–3589.

    Article  CAS  Google Scholar 

  14. Evans MJ, Kaufman MH . Establishment in culture of pluripotential cells from mouse embryos. Nature 1981; 292: 154–156.

    Article  CAS  Google Scholar 

  15. Martin GR . Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci USA 1981; 78: 7634–7638.

    Article  CAS  Google Scholar 

  16. Doetschman T, Gregg RG, Maeda N et al. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 1987; 330: 576–578.

    Article  CAS  Google Scholar 

  17. Thomas KR, Capecchi MR . Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 1987; 51: 503–512.

    Article  CAS  Google Scholar 

  18. Wilmut I, Schnieke AE, McWhir J et al. Viable offspring derived from fetal and adult mammalian cells. Nature 1997; 385: 810–813.

    Article  CAS  Google Scholar 

  19. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. Embryonic stem cell lines derived from human blastocysts. Science 1998; 282: 1145–1147.

    Article  CAS  Google Scholar 

  20. Shamblott MJ, Axelman J, Wang S et al. Derivation of pluripotent stem cells from cultured human primordial germ cells. Proc Natl Acad Sci USA 1998; 95: 13726–13731.

    Article  CAS  Google Scholar 

  21. Committee on Science, Engineering, and Public Policy. Scientific and Medical Aspects of Human Reproductive Cloning. National Academy Press: Washington, DC, 2002. http://www.nap.edu/catalog/10285.html

  22. Bjornson CR, Rietze RL, Reynolds BA et al. Turning brain into blood: a hematopoietic fate adopted by adult neural stem cells in vivo. Science 1999; 283: 534–537.

    Article  CAS  Google Scholar 

  23. Anderson DJ, Gage FH, Weissman IL . Can stem cells cross lineage boundaries? Nature Med 2001; 7: 393–395.

    Article  CAS  Google Scholar 

  24. Wurmser AE, Gage FH . Stem cells: cell fusion causes confusion. Nature 2002; 416: 485–487.

    Article  CAS  Google Scholar 

  25. Morshead CM, Benveniste P, Iscove NN et al. Hematopoietic competence is a rare property of neural stem cells that may depend on genetic and epigenetic alterations. Nat Med 2002; 8: 268–273.

    Article  CAS  Google Scholar 

  26. Department of Health and Human Services. Stem cells: Scientific Progress and Future Research Directions. National Institutes of Health, 2001 http://www.nih.gov/news/stemcell/scireport.htm

  27. Zerhouni E . Stem cell research. Senate Appropriations Subcommittee on Labor. HHS, Education: Washington, DC, 2002 http://www.nih.gov/about/director/092502sctestimony.htm

    Google Scholar 

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Spangrude, G. When is a stem cell really a stem cell?. Bone Marrow Transplant 32 (Suppl 1), S7–S11 (2003). https://doi.org/10.1038/sj.bmt.1703936

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