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Dendritic Cells

Dendritic cells can be successfully generated from CD34+ cord blood cells in the presence of autologous cord blood plasma

Bone Marrow Transplantation volume 26, pages 371–376 (2000)Cite this article

Abstract

Dendritic cells (DCs) are currently being considered as adjuvants in immunotherapy. Depending on their source and culture conditions, they show different features and maturation states. Dendritic cells can be generated from monocytes and CD34+ haematopoietic stem cells, from both adult and cord blood. Here, we report the generation of mature DCs from enriched CD34+ cord blood (CB) cells using autologous cord blood plasma (ACBP) as a source of serum proteins and factors. In the presence of ACBP, CD34+ cells proliferated and differentiated resulting in a population of cells with a dendritic phenotype as assessed by morphology and flow cytometry analyses. The DC population obtained using ACBP showed higher levels of HLA class II molecules, co-stimulatory molecules including CD40, CD80 or CD86, and the dendritic cell marker CD83, compared with those generated in adult blood serum (ABS). Furthermore, the DCs generated in the presence of ACBP were more potent stimulatory cells in the mixed lymphocyte:dendritic cell reactions (MLDCR), compared to cells generated in ABS. Similar results were obtained using homologous cord blood plasma (HCBP). These results show that ACBP can support the generation of DCs from CD34+ progenitor cells when only GM-CSF and TNFα are used as differentiating cytokines. Bone Marrow Transplantation (2000) 26, 371–376.

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References

  1. Steinmann RM . The dendritic cell system and its role in immunogenicity Annu Rev Immunol 1991 9: 271–296

    Article  Google Scholar 

  2. Romani N, Koide S, Crowley M et al. Presentation of exogenous protein antigens by dendritic cells to T cell clones: intact protein is presented best by immature epidermal Langerhans cells J Exp Med 1989 169: 1169–1178

    Article  CAS  PubMed  Google Scholar 

  3. Steinmann RM, Hoffman L, Pope M . Maturation and migration of cutaneous dendritic cells J Invest Dermatol 1995 105: 2S–7S

    Article  Google Scholar 

  4. Pick WF, Majdic O, Kohl P et al. Molecular and functional characteristics of dendritic cells generated from highly purified CD14+ peripheral blood monocytes J Immunol 1996 157: 3850–3859

    Google Scholar 

  5. Cella M, Scheidegger D, Palmer-Lehmann K et al. Ligation of CD40 on dendritic cells triggers production of high levels of interleukin 12 and enhances T cell stimulatory capacity: T-T help via APC presentation J Exp Med 1996 184: 747–752

    Article  CAS  PubMed  Google Scholar 

  6. Koch F, Stanzl U, Jennewein P et al. High level IL-12 production by murine dendritic cells: up-regulation via MHC class II and CD40 molecules and down-regulation by IL-4 and IL-10 J Exp Med 1996 184: 741–746

    Article  CAS  PubMed  Google Scholar 

  7. Hart DNJ . Dendritic cells: unique leukocyte populations which control primary immune response Blood 1997 90: 3245–3287

    CAS  PubMed  Google Scholar 

  8. Sallusto F, Lanzavecchia A . Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte–macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumour necrosis factor alpha J Exp Med 1994 179: 1109–1118

    Article  CAS  PubMed  Google Scholar 

  9. Romani N, Reider D, Heuer M et al. Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability J Immunol Methods 1996 196: 137–151

    Article  CAS  PubMed  Google Scholar 

  10. Reddy A, Sapp M, Feldman M et al. A monocyte conditioned medium is more effective than defined cytokines in mediating the terminal maturation of human dendritic cells Blood 1997 90: 3640–3646

    CAS  PubMed  Google Scholar 

  11. Herbst B, Kohler G, Mackensen A et al. CD34+ peripheral blood progenitor cell and monocyte derived dendritic cells: a comparative analysis Br J Haematol 1997 99: 490–499

    Article  CAS  PubMed  Google Scholar 

  12. Caux C, Vanbervliet B, Massacrier C et al. CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF + TNFα J Exp Med 1996 184: 695–706

    Article  CAS  PubMed  Google Scholar 

  13. Saraya K, Reid CD . Stem cell factor and the regulation of dendritic cell production from CD34+ progenitors in bone marow and cord blood Br J Haematol 1996 93: 258–264

    Article  CAS  PubMed  Google Scholar 

  14. Maraskovsky E, Brasel K, Teepe M et al. In vivo administration of flt3 ligand results in generation of large numbers of dendritic cells in the lymphoid tissue of mice J Exp Med 1996 184: 1953–1962

    Article  CAS  PubMed  Google Scholar 

  15. Arrighi JF, Hauser C, Chapuis B et al. Long-term culture of human CD34+ progenitors with Flt3 ligand, thrombopoietin and SCF induces extensive amplification of a CD34− CD14− and a CD34− CD14+ dendritic cell precursor Blood 1999 93: 2244–2252

    CAS  PubMed  Google Scholar 

  16. Geissmann F, Prost C, Monnet JP et al. Transforming growth factor β1, in the presence of granulocyte/macrophage colony-stimulating factor and interleukin 4, induces differentiation of human peripheral blood monocytes into dendritic Langerhans cells J Exp Med 1998 187: 961–966

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Romani N, Gruner S, Brang D et al. Proliferating dendritic cell progenitors in human blood J Exp Med 1994 180: 83–93

    Article  CAS  PubMed  Google Scholar 

  18. Schuler G, Steinmann RM . Dendritic cells as adjuvants for immune-mediated resistance to tumors J Exp Med 1997 186: 1183–1187

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Menetrier-Caux C, Montmain G, Dieu MC et al. Inhibition of the differentiation of dendritic cells from CD34+ progenitors by tumor cells: role of interleukin 6 and macrophage colony-stimulating factor Blood 1998 92: 4778–4791

    CAS  PubMed  Google Scholar 

  20. Steinbrink K, Jonuleit H, Muller G et al. Interleukin 10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8+ T cells resulting in failure to lyse tumor cells Blood 1999 93: 1634–1642

    CAS  PubMed  Google Scholar 

  21. Eljaafari A, Duperrier K, Mazet S et al. Generation of stable monocyte-derived dendritic cells in the presence of high concentrations of homologous or autologous serum: influence of extra-cellular pH Hum Immunol 1998 59: 625–634

    Article  CAS  PubMed  Google Scholar 

  22. Rosenzwajg M, Camus S, Guigon M, Gluckman JC . The influence of interleukin (IL)-4, IL-13 and Flt3 ligand on human dendritic cell differentiation from cord blood CD34+ progenitor cells Exp Hematol 1998 26: 63–72

    CAS  PubMed  Google Scholar 

  23. Rosenzwajg M, Gluckman JC . Problems raised by the in vitro generation of dendritic cells for use in immunotherapy Exp Hematol 1999 27: 387–388

    Article  CAS  PubMed  Google Scholar 

  24. Nicola M, Longoni P, Magni M, Gianni AM . The influence of interleukin (IL)-4, IL-13 and Flt3 ligand on human dendritic cell differentiation from cord blood CD34+ progenitor cells. The importance of the source of serum Exp Hematol 1998 27: 386–387

    Google Scholar 

  25. Sherr CJ . Colony stimulating factor-1 receptor Blood 1990 75: 1–12

    CAS  PubMed  Google Scholar 

  26. Praloran V, Coupey L, Donnard M et al. Elevation of serum M-CSF concentrations during pregnancy and ovarian hyperstimulation Br J Haematol 1994 86: 675–677

    Article  CAS  PubMed  Google Scholar 

  27. Saito S, Saito M, Enomoto M et al. Human macrophage colony stimulating factor induces differentiation of trophoblasts Growth Factors 1993 9: 11–19

    Article  CAS  PubMed  Google Scholar 

  28. Ratta M, Rondelli D, Fortuna A et al. Generation and functional characterisation of human dendritic cells derived from CD34 cells mobilised into peripheral blood: comparison with bone marrow CD34+ cells Br J Haematol 1998 101: 756–765

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Histocompatibility and Immunogenetics, NBS, London and The South East, North London Centre, London, UK

    FE Borràs, NC Matthews, R Patel & C Navarrete

  2. Department of Immunology, Royal Free and University College Medical School, London, UK

    C Navarrete

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  1. FE Borràs
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  2. NC Matthews
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  3. R Patel
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  4. C Navarrete
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Borràs, F., Matthews, N., Patel, R. et al. Dendritic cells can be successfully generated from CD34+ cord blood cells in the presence of autologous cord blood plasma. Bone Marrow Transplant 26, 371–376 (2000). https://doi.org/10.1038/sj.bmt.1702525

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