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Oral health

Salivary lipid changes in young adult tobacco smokers and e-cigarette users: a hidden risk to oral health?

Evidence-Based Dentistry (2024)Cite this article

Subjects

Abstract

Study design

A cross-sectional, age- and gender-matched study was conducted to investigate the effects of different forms of nicotine delivery on salivary lipid profiles among young adult novice smokers compared to non-smokers.

Objective

To assess the effect of smoking traditional cigarettes, e-cigarettes, and heated tobacco products (HTPs) on the levels of specific sphingolipids (sphingosine, sphinganine, and sphingosine-1-phosphate), various ceramides, and lipid peroxidation products [malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE)] in both unstimulated and stimulated saliva samples collected from healthy young adults who had been smoking for 1-3 years and used only 1 of the 3 nicotine delivering methods.

Methods

Selection criteria included healthy young adults under 30 years old, with normal BMI and typical diet composition, and with no oral inflammatory lesions, orthodontic/dental appliances, or recent intake of medications or supplements. A total of 75 smokers and 25 non-smokers were enrolled in the study. Smokers were categorized into three groups, each comprising 25 individuals: traditional cigarette smokers, e-cigarette users, and HTPs smokers. Saliva samples were collected and analyzed for sphingolipid concentrations using ultra-high-performance liquid chromatography-tandem mass spectrometry. The concentrations of MDA and 4-HNE were measured using colorimetric and ELISA assays, respectively.

Results

The average smoking intensity in the traditional cigarette group was 10 cigarettes per day. Salivary sphingolipid and ceramides concentrations were significantly lower in smokers compared to non-smokers across all nicotine delivery methods (p < 0.0001). Moreover, traditional cigarette smokers exhibited higher levels of 4-HNE and MDA in both stimulated and unstimulated saliva, compared to non-smokers (p < 0.01). In stimulated saliva, both MDA and 4-HNE in e-cigarette users, and MDA in HTPs users, showed significantly lower concentrations than their comparators in traditional cigarette smokers (p < 0.01).

Conclusion

Different nicotine delivery methods impact salivary lipid profile during the initial period of smoking habit. Reduced sphingolipids and elevated lipid peroxidation products suggest a disturbed lipid balance in the oral cavity due to enhanced oxidative stress within the salivary glands of novice smokers.

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References

  1. Fineide F, Chen X, Bjellaas T, Vitelli V, Utheim TP, Jensen JL, et al. Characterization of lipids in saliva, tears and minor salivary glands of Sjögren’s syndrome patients using an HPLC/MS-based approach. Int J Mol Sci. 2021;22:8997.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Slomiany BL, Murty VL, Slomiany A. Salivary lipids in health and disease. Prog Lipid Res. 1985;24:311–24.

    Article  CAS  PubMed  Google Scholar 

  3. Matczuk J, Żendzian-Piotrowska M, Maciejczyk M, Kurek K. Salivary lipids: a review. Adv Clin Exp Med. 2017;26:1021–9.

    Article  PubMed  Google Scholar 

  4. Guentsch A, Preshaw PM, Bremer-Streck S, Klinger G, Glockmann E, Sigusch BW. Lipid peroxidation and antioxidant activity in saliva of periodontitis patients: effect of smoking and periodontal treatment. Clin Oral Investig. 2008;12:345–52.

    Article  PubMed  Google Scholar 

  5. Majid OW. Dose-response association of smoking with delayed healing of apical periodontitis after endodontic treatment. Evid Based Dent. 2023;24:174–5.

    Article  PubMed  Google Scholar 

  6. Majid OW. Further evidence confirms the association between smoking and dry socket: a motivational opportunity for tobacco cessation. Evid Based Dent. 2023;24:181–3.

    Article  PubMed  Google Scholar 

  7. Majid OW. Gunshot injuries to the parotid gland: Patterns of injury and primary management. Br J Oral Maxillofac Surg. 2007;45:571–2.

    Article  PubMed  Google Scholar 

  8. Palmerini CA, Saccardi C, Ferracci F, Arienti S. Lipid patterns in the saliva of smoking young adults. Hum Exp Toxicol. 2011;30:1482–8.

    Article  CAS  PubMed  Google Scholar 

  9. Kurtul N, Gökpınar E. Salivary lipid peroxidation and total sialic acid levels in smokers and smokeless tobacco users as Maraş powder. Mediators Inflamm. 2012;2012:619293.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Zięba S, Błachnio-Zabielska A, Maciejczyk M, Pogodzińska K, Szuta M, Lo Giudice G, et al. Impact of smoking on salivary lipid profile and oxidative stress in young adults: a comparative analysis between traditional cigarettes, E-cigarettes, and heat-not-burn products. Med Sci Monit. 2024;30:e942507.

    PubMed  PubMed Central  Google Scholar 

  11. Maceyka M, Spiegel S. Sphingolipid metabolites in inflammatory disease. Nature. 2014;510:58–67.

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  12. Majid OW. Dose- and time-dependent association of smoking and its cessation with risk of peri-implant diseases. Evid Based Dent. 2024;25:15–16.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Mosul University, Mosul, Iraq

    Omer Waleed Majid

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  1. Omer Waleed Majid
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Correspondence to Omer Waleed Majid.

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Majid, O.W. Salivary lipid changes in young adult tobacco smokers and e-cigarette users: a hidden risk to oral health?. Evid Based Dent (2024). https://doi.org/10.1038/s41432-024-00998-5

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  • DOI: https://doi.org/10.1038/s41432-024-00998-5

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