Abstract
A systematic study of the secondary metabolites of the wild granaticin-producing strain Streptomyces vietnamensis GIMV4.0001 led to the isolation of six known early shunt products related to actinorhodin, SEK34 (3), SEK34b (4), mutactin (5), dehydromutactin (7), EM18 (8) and GTRI-02 (9). While the other shunt products were minor or trace products, the production ratio of SEK34 (3) and SEK34b (4) to granaticins was strikingly high. Nearly 64% of the intermediate with the first ring closed went to the SEK34/SEK34b aberrant pathway. The high level of the aberrant metabolic flow toward the early shunt products SEK34 and SEK34b indicated that the second ring closure of the granaticin (1) biosynthesis is a key limiting step in the granaticin biosynthetic machinery of S. vietnamensis GIMV4.0001.
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Acknowledgements
This work was supported, in part, by the National Natural Science Foundation of China (Grant No. 31470188), the Guangdong Science and Technology Program (Grant Nos. 2015A020211022 and 2016A010105013), the Guangdong Province Science and Technology Innovation Strategy Special Fund (Grant No. 2018B020206001) and GDAS' Project of Science and Technology Development (2019GDASYL-0401002). The authors are grateful to Dr Hao Zhou at the Yunnan University and Dr. Liao-Bin Dong at the Scripps Research Institute for helps in structure elucidation.
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Deng, MR., Li, Y., He, HH. et al. An aberrant metabolic flow toward early shunt products in the granaticin biosynthetic machinery of Streptomyces vietnamensis GIMV4.0001. J Antibiot 73, 260–264 (2020). https://doi.org/10.1038/s41429-019-0267-8
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DOI: https://doi.org/10.1038/s41429-019-0267-8