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Lymphoma

Genomic characterization of diffuse large B-cell lymphoma transformation of nodular lymphocyte-predominant Hodgkin lymphoma

Leukemia volume 34, pages 2238–2242 (2020)Cite this article

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Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is a rare subtype of lymphoma that usually has an indolent clinical behavior. Similar to classical Hodgkin lymphoma (cHL), the tumor cells in NLPHL represent a minority of the cells in the tumor and, therefore, have been a challenge to study because of insufficient material for analysis. Transformation of NLPHL (tNLPHL) to diffuse large B-cell lymphoma (DLBCL) is seen in up to 30% of the cases [1] and can have varying morphologic patterns. However, two main patterns are seen, T-cell/histiocyte-rich large B-cell lymphoma (THRLBCL) and more typical DLBCL with sheets of large cells. Because the tumor cells are also sparse in THRLBCL, it has also been difficult to study these cases. However, with laser capture microdissection, a limited number of cases of tNLPHL have been investigated by next-generation sequencing [2, 3] using limited gene panels.

In this study, we evaluated tNLPHL cases that have the sheet-like growth pattern typical of de novo DLBCL. We characterized the genomic landscape of such cases using next-generation sequencing and copy number analysis (CNA) to gain a better understanding of the pathogenesis of these tumors.

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Fig. 1: Copy number analysis of 18 cases of transformed nodular lymphocyte-predominant Hodgkin lymphoma.
Fig. 2: Select copy number abnormalities and mutation analysis of 19 cases (8 paired and 11 unpaired) of tNLPHL.

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Authors and Affiliations

  1. Department of Pathology, City of Hope National Medical Center, Duarte, CA, USA

    Joo Y. Song, Qiang Gong, Victoria Bedell, Maria Valle-Catuna, Joyce Murata-Collins, Dennis D. Weisenburger & Wing C. Chan

  2. Hematopathology Section, Laboratory of Pathology, National Cancer Institute, Bethesda, MD, USA

    Caoimhe Egan & Elaine S. Jaffe

  3. Department of Pathology, University of Nebraska, Omaha, NE, USA

    Alyssa C. Bouska, Weiwei Zhang & Javeed Iqbal

  4. Department of Pathology, University of Chicago, Chicago, IL, USA

    Girish Venkataraman

  5. Department of Hematology & Hematopoietic Cell Transplantation, City of Hope National Medical Center, Duarte, CA, USA

    Alex F. Herrera

  6. Department of Information Sciences, City of Hope National Medical Center, Duarte, CA, USA

    Lu Chen

  7. Department of Diabetes and Cancer Discovery Science, City of Hope National Medical Center, Duarte, CA, USA

    Rebecca Ottesen & Joyce C. Niland

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  1. Joo Y. Song
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Contributions

JYS and WCC designed, performed the research, analyzed the data, and wrote the paper. CE, ACB, WZ, QG, JI, GV, AFH, DDW, and ESJ analyzed the data and edited the paper. LC, JCN, and RO provided the statistical analysis and edited the paper. VB, MV-C, and JM-C performed the research and edited the paper. All authors reviewed the paper. Research reported in this publication included work performed in the Pathology Core and Integrated Genomics Core supported by the National Cancer Institute of the National Institutes of Health under grant number P30CA033572. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Correspondence to Joo Y. Song.

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Song, J.Y., Egan, C., Bouska, A.C. et al. Genomic characterization of diffuse large B-cell lymphoma transformation of nodular lymphocyte-predominant Hodgkin lymphoma. Leukemia 34, 2238–2242 (2020). https://doi.org/10.1038/s41375-020-0739-7

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