Abstract
Background: Hyperoxia is one of the risks to cause BPD or ROP in preterm infants. It has been known to suppress the angiogenesis of vascular endothelial cells. However, the effect of hyperoxia to the antoxienzyme activity of vascular endothelial cells is not very clear.
Objective: To evaluate the effects of hyperoxia to the superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities of cultured pulmonary arterial endothelial cells (PAEC).
Materials and Methods: Primary fetal sheep (GA 125 to 140 days) pulmonary arterial endothelial cells (PAEC) were cultured under room air, 60%, and 80% oxygen. NO donor (DETA NONOate) was administrated to the culture medium with the concentrations of 0, 0.01, 0.02, and 0.05mM. The After 72 hours, the cells were collected and lysed. Protein concentrations, Cu/Zn-SOD and cellular GPx activities were measured by spectrophotometer.
Results: The SOD activity was highest under 60% oxygen concentration. However, the activity range was very large. The cellular GPx activity was highest under room air. The activity decreased under 60% and 80% oxygen concentrations. Exogenous nitric oxide may decrease the apoptosis percentage of PAEC under 60% oxygen concentration. However, it didn't affect antioxienzyme activity of PAEC under hyperoxic condition.
Conclusion: Hyperoxia suppressed cellular GPx activity in PAEC. This effect might contribute to the development of BPD in preterm infants.
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Fu, RH., Cho, YH., Lien, R. et al. 493 Hyperoxia Suppress Antioxienzyme Activity in Cultured Pulmonary Arterial Endothelial Cells. Pediatr Res 68 (Suppl 1), 252–253 (2010). https://doi.org/10.1203/00006450-201011001-00493
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DOI: https://doi.org/10.1203/00006450-201011001-00493