Abstract
Purpose. We investigated the effects of immuoglobulin(IG) upon experimental giant cell myocarditis in rats with an analysis of the phenotypic characteristic of myocardial dendritic cell(DC) and ribonuclease protein assay(RPA) for myocardial cytokines. Also, we investigated which fragment of IG, F(ab')2 or Fc, was effetive to ameliorate myocarditis. Methods. Giant cell myocarditis was induced in rats by immunization of porcine cardiac myosin. Human intact IG(1g/kg/day) or human F(ab')2 were administered intraperitoneally everyday until 21st day. Immunohistochemistry was performed to analyze the immunological behavior of DC and other infliltrating cells. In addition, we performed the RPA to confirm the anti-inflammatory action of IG. Results. Intact IG therapy significantly ameliorated experimental giant cell myocarditis macroscopically and microscopically, but F(ab')2 fragments did not ameliorate significantly. Immunohistochemichal analysis showed that intact IG therapy suppressed DC expression both during the early and the fulminant phases. The RPA analysis revealed that intact IG therapy completely suppressed the mRNA expressions of interleukin(IL)-1, IL-6, and interferon-γ. Conclusions. The present study provides the evidence that IG therapy suppressed giant cell myocarditis due to the suppression of DC expression and the anti-inflammatory actions, and that these effects may be mediated via the Fc portion of IG.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kishimoto, C., Shioji, K., Yuan, Z. et al. Therapy with Immuoglobulin Ameliorates Giant Cell Myocarditis in Rats. Pediatr Res 53, 175 (2003). https://doi.org/10.1203/00006450-200301000-00127
Issue Date:
DOI: https://doi.org/10.1203/00006450-200301000-00127