Abstract
Aim: To further explore the pathogenesis of Kawasaki disease (KD). Methods: Observing cell morphological change, Calculating percentage of apoptotic cells and assaying DNA fragmentation in peripheral blood mononuclear cell (PBMC) stimulated by anti-CD3 for 0, 12, 24,48,72h in Vitro from 30 KD patients and 20 age-matched health children. Results: Apoptotic cell percentage and DNA fragmentation were markedly decreased (P<0.001) and delayed(in comparison with those in normal controls),which could reach the states of control group only by 72h culture. Remarkably increased production of IL-6 in cultured supernatants of PBMC induced by phytohemagglutinin (PHA) from KD patients was found in comparison with those of PBMC from controls (P<0.001). Adding anti-IL-6mAb to the cultures or using intravenous immunoglobulin (IVIG) in Vivo led to significantly decreased production of IL-6 (P<0.001),and decreased apoptotic cell percentage and delayed DNA fragmentation reversed to be compatible with normal controls. Conclusion: Peripheral blood lymphocyte apoptosis is inhibited in KD and over-produced IL-6 might be involved in this process.
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Yi, Q., Li, C. & Yang, X. The Effect of IL-6 on Peripheral Blood Lymphocyte Apoptosis in Acute Kawasaki Disease. Pediatr Res 53, 170 (2003). https://doi.org/10.1203/00006450-200301000-00100
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DOI: https://doi.org/10.1203/00006450-200301000-00100