The purpose of this study was to assess the freguency of positive antinuclear (ANA) and anti dsDNA (αDNA) antibodies among healthy Greek peadiatric population. ANA and αDNA antibodies were determined in the sera of 325 Greek infants and children of both gender, aged 2 months to 14 years. The children were admitted at “Aghia Sophia” Teaching Children's Hospital for surgical correction of minor anatomical abnormalities, but they were otherwise healthy. Autoantibodies were detected by indirect immunofluorescense using HEP2 for ANA and Crithidia Luciliae for αDNA. The initial serum dilution was 1:40 for ANA and 1:10 for αDNA. In case of positive ANA titers, serial dilutions were undertaken. Twenty four sera were positive for ANA but none for αDNA antidodies. The percantage of children with positive ANA titers was statistically significantly higher among older children (>5years old) than the younger ones. The children who had an ANA titer over 1:40 are, still now, all in good clinical condition and under investigation. Comments on indicated policies for medical attendance of healthy children with high ANA titers are made.