Abstract
We provide evidence for high-level macrophage infiltration early in the course of RDS by both sequential bronchoalveolar lavage (BAL) in 38 infants (13 BPD, 17 RDS and 8 controls) and immunohistochemical analysis of 45 post-mortem specimens.
Using lineage-specific markers we have shown that the majority of cells obtained by BAL after 48 hours are of macrophage origin, and most are immunoreactive for TNFα. High level production of the macrophage-associated chemokine MIP-1α and the cytokines TNFα and IL-1 were found from day 1 in RDS and BPD infants. The infants who developed BPD had significant elevation of inflammatory cell numbers and cytokine levels compared to those with an uncomplicated course. Dexamethasone significantly decreased all parameters within 24 hours.
Post-mortem specimens demonstrated interstitial infiltration of macrophages, polymorphs and TNFα+ cells, maximal by 72 hours, associated with major disruption of sulphated glycosaminoglycans (GAGs), molecules of central importance in the control of albumin flux and tissue compliance (Lancet 1993:.3 41: 711-14).
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Murch, S., Macdonald, T. & Costeloe, K. 171 MACROPHAGE INFILTRATION AND MATRIX DISRUPTION IN∼RDS. Pediatr Res 36, 31 (1994). https://doi.org/10.1203/00006450-199407000-00171
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DOI: https://doi.org/10.1203/00006450-199407000-00171