Abstract
Lung fibroblast proliferation is an important component of diseases such as bronchopulmonary dysplasia. A possible mechanism contributing to this phenomenon could be the loss of inhibitory influences by the adjacent epithelial layer, which is damaged early in the course of the disease.
In the present study the influence of rat type-II cell-conditioned medium (CM) on the proliferation of human fetal fibroblasts was measured.
Methods: CM from type II cells cultured in 1 or 10% fetal calf serum for 1 day (1%ld; 10%ld) or 3 days (1%3d; 10%3d) were added (1:10) to fibroblast cultures.
Fibroblast proliferation was measured by incorporation of bromodeoxyuridine (BrdU), as estimated by ELISA.
Results: CM 1%ld inhibited incorporation of BrdU by 35% (p<0.05), CM 10%ld by 24% (p<0.05) of control (no CM). CM 1%3d stimulated the incorporation of BrdU by 8% (n.s.), CM 10%3d by 13% (p<0.05) above control.
Conclusions: Freshly prepared type-II cells in culture inhibit the proliferation of human fetal fibroblasts.
After 3 days in culture this property is lost, and may be replaced by stimulatory influences.
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Stevens, P., Rey, M. & Meienreis-Sudau, V. INFLUENCE OF TYP-II CELL-CONDITONED MEDIUM ON THE PROLIFERATION OF FIBROBLAST CELL CULTURES. Pediatr Res 32, 636 (1992). https://doi.org/10.1203/00006450-199211000-00190
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DOI: https://doi.org/10.1203/00006450-199211000-00190