Abstract
In ischemia-reperfusion injury. ROM are produced and may cause tissue damage followed by fibrosis. Many cells types may be injured but ECs are the most vulnerable. We studied possible differences In the sensitivity to ROM between human fetal FBs and ECs.
Cells were labelled overnight with 14C-adenine, washed and further incubated with either H2O2 or with xanthine oxidase (XO) and hypoxanthine (Hx). Nucleotides from cells and medium as well as catabolic products (Hx, xanthine and urate) from medium were separated and counted. Nucleotide depletion (or % of initial cpm remaining) is a sensitive index of cell damage.
FBs incubated with XO (80 mU/ml) and Hx (100 μM) for 4h retained 73±2% of cpm in cell nucleotides (control 85±4%), the rest appearing in catabolic products, whereas EC nucleotides were nearly totally depleted (11±2% remaining vs. 78±2% in controls). H2O2 at 20 μM for 10 or 30 min, or 100 μM for 10 min. did not deplete nucleotides from FBs, (92±2%, 92±5% and 88±5% remaining, respectively, vs. control 30 min 91±2%), while the corresponding figures for ECs were 74±9%, 55±9% and 48±7%, controls 80 ±3%. H2O2 at 100 μM for 30 min had a slight effect in FBs, (76±8%) but stronger in ECs (36126%).
We conclude that FBs survive ROM-induced damage better than ECs and are thus able to proliferate in the reparative stage of tissue injury.
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Aalto, T., Raivio, K. SENSITIVITY TO REACTIVE OXYGEN METABOLITES (ROM) OF HUMAN FIBROBLASTS (FB) COMPARED WITH ENDOTHELIAL CELLS (EC). Pediatr Res 32, 636 (1992). https://doi.org/10.1203/00006450-199211000-00189
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DOI: https://doi.org/10.1203/00006450-199211000-00189