Abstract
Previous investigations have shown that cord blood PMNs produce more O−2 than adults cells when stimulated with a chemotactic peptide (FMLP) and a priming effect of labor was advocated. However little is known about O−2 production rate by neonatal PMNs. We have measured the O−2 production in PMNs from a) cord blood of 15 vaginally delivered healthy term neonates, b) cord blood of 7 vaginally delivered and moderately distressed neonates, c) blood of 16 healthy term neonates aged 4-5 days and d) blood of 8 adults. In 5 cases blood was taken twice, from the cord and the neonate 4 days later. The O−2 was measured using 10−7M FMLP as a PMNs stimulus.
Results: An increased O2 generation (nmol O−2/5×105 cells) from neonatal PMNs (13±2,6) compared to that of cord (11.4±1.7 p<0.05) and of adult (7.8±1.6 p<0.001) was found. This increase was confirmed in the 5 cases of paired blood samples (cord 10.8±2.1 and neonate 14± 2.7). PMNs from cord of distressed neonates (9.0±1.8) produce less O−2 that those of nonstressed neonates (11.4±1.7 p<0.05).
Conclusion: It appears that during labor a depressive effect on neonatal PMNs is exerted and this effect is more pronounced in cases of complicated labor. This may be related to the severity of perinatal infections especially in the asphyxiated neonates.
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Mandyla, H., Koliou, M., Kostantzas, N. et al. PRODUCTION OF SUPEROXIDE ANION (O−2) BY NEUTROPHILS (PMNS) FROM CORD, NEONATAL AND ADULT BLOOD. Pediatr Res 32, 630 (1992). https://doi.org/10.1203/00006450-199211000-00153
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DOI: https://doi.org/10.1203/00006450-199211000-00153