Abstract
Diagnosis of allergy is supported by “in vivo” (ex: SPT) and “in vitro” methods (ex: specific IgE). SPT is influenciated by a lot of variables, so standardization is essential to obtain precise and reproducible quantitative results. This can be a achieved by a method ol reading using a computerized digitalized graphics tablet with CAD software, created and validated by the authors and published elsewhere. The Pharmacia CAP System allows precise quantification of specific IgE and is more sensitive than RAST. Preliminar data obtained by the authors, showed that for D. Pteronyssinus (D.Pt) the SPT results done with extracts from Bencard (B) and Merck (M) had good correlation with specific IgE determined by CAP System (as quantitative values and as +/-).
The aim of the study was to establish ihe possible relations between quantitative results of SPT and CAP and different respiratory allergic diseases with the same severity in a pediatric population sensitized to D.Pt. We include 73 children (47 ♂ 26 ♀,) aged 4 to 15 y. (mean age ± SD:9.70 ± 3.24), 13 with Rhinitis (R), 16 with Asthma (A) and 44 with A+R. The SPT was done with a standardized lancet (DHS) using cut oil +/- 7mm2, with Ihe method cited above and with extracts for D. PI from B and M. The determination of specific IgE was done by Pharmacia CAP System.
Results: 1) Mean age ± SD in R group : 11.31 ± 2.72; A group: 9.48 ± 3.50, without statistically significant dilferencies. 2) SPT results (mean areas ± SD): R group (B:43.20 ± 20.69 mm2; M: 36.72 ± 22.18mm2); A group (B:50.48 ± 25.11mm2; M: 50.70 ± 35.24mm2); A+R group (B: 61.93 ± 28.20mm2; M: 47.98 ± 39.34mm2). 3) CAP results (mean values ± SD): R group (13.92 ± 14.44KU/L; A group (27.01 ± 30.26KU/L); A+R group (39.12 ± 29.35KU/L).
Conclusions: 1) The results of quantitative SPT for both extracts related lo R, A and A+R don't show statistically signilicant ditterencies. 2) The quantitative values of specilic IgE show that there are differencies between all the entities, with lower results to R and higher to A+R. 3) Based on previous data suggesting that within the same disease, the severity is directed related with higher titers of specific IgE, our data may support that the “severity” of A+R > A > R.
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Almeida, M., Marta, C., Pinto, P. et al. QUANTITATIVE MEASUREMENTS OF SKIN PRICK TESTS (SPT) AND SPECIFIC IGE - RELATION BETWEEN VALUES AND DIFFERENT RESPIRATORY ALLERGIC DISEASES. Pediatr Res 32, 609 (1992). https://doi.org/10.1203/00006450-199211000-00028
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DOI: https://doi.org/10.1203/00006450-199211000-00028