Abstract
Polyunsaturated fatty acids [PUFAs] of low density lipoproteins ILDL) are protected against LPO by vitamin E and carotenoids. In vitro exposure of LDL to a prooxidant leads to consumption of these antioxidants [AO] (lag-phase [lag]), resulting in formation of conjugated dienes [CD]. In 5 CF patients CD generation in LDL was monitored up to complete LDL oxidation. Lag wan measured, α-, γ-tocopherol [T], β-carotene [c] and other carotenoids were determined in plasma and LDL. 3 patients showed a short lag (30, 57, 60 min) compared to others (135, 152 min) and to healthy controls, indicating a suboptimal supply of AO. They also had decreased α-T in plasma (median) (11-0 μmol/l) and LDL (1.4 nmol/mg). β-C was very low in all patients (0.08 μmol/l), as were other carotenoids. The length of the lag was more closely related to LDL α-T (r2=0.84) than in controls (r2=0.51), indicating the lack of other AO. CD content in fully oxidized LDL was considerably lower in patients (CD absorbance 0.4-0.6) compared to controls (0.8-1.0), suggesting a limited amount of oxidizable PUFAs in LDL. For identical LDL α-T CF patients had a longer lag than controls, despite lower carotenoids. In conclusion, LDL-LPO in CF is almost exclusively prevented by α-T. Low oxidizable PUFAs in LDL due to essential FA deficiency lead to limited CD formation even in severe AO deficiency.
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Winklhofer-Roob, B., Puhl, U., Shmerling, D. et al. 71 DETERMINANTS OF LIPID PEROXIDATION [LPO] IN CYSTIC FIBROSIS [CF]. Pediatr Res 30, 640 (1991). https://doi.org/10.1203/00006450-199112000-00101
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DOI: https://doi.org/10.1203/00006450-199112000-00101