Abstract
Adhesion of leukocytes to the microvascular endothelium is a common feature in disorders such as ischemia-reperfusion injury and complications of bone marrow transplantation. Using intravital fluorescence microscopy in a skinfold chamber model in hamsters, we found that injection of human LDLox initiated leukocyte adhesion to microvascular endothelium while native LDL did not (p<0.01). After a fish oil diet, arachidonic acid (AA) was largely replaced by eicosapentaenoic acid (EPA) in leukocytes, and the adhesion effect of LDLox was inhibited (p<0.01). Leukotriene (LT) B4, the lipoxygenase product of AA, is a potent chemotactic mediator, whereas LTB5, the EPA product, is weakly chemotactic. We suggest that LDLox initiate adhesion of leukocytes to microvascular endothelium by promoting LTB4 synthesis and that fish oil diet inhibits this LDLox effect.
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Hüber, C., Lehr, HA., Messmer, K. et al. 64 OXIPATIVELY MODIFIED HUMAN LOW DENSITY LIPOPROTEINS (LDLox ) INDUCE LEUKOCYTE ADHESION TO VASCULAR ENDOTHELIUM OF HAMSTERS IN VIVO. FISH OIL DIET INHIBITS THIS EFFECT. Pediatr Res 30, 638 (1991). https://doi.org/10.1203/00006450-199112000-00094
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DOI: https://doi.org/10.1203/00006450-199112000-00094