Abstract
We have previously shown that children with aCAH have circulating activated T lymphocytes specifically sensitised to liver cell membrane, which are involved in mediating liver damage. To determine the frequency of liver membrane specific T lymphocytes (LMSTL) amongst circulating T cells we have established limiting dilution cultures of peripheral blood mononuclear cells from 6 children with aCAH and 6 age matched normal controls. Proliferation of purified T cells, cultured with irradiated autologous B cells as antigen presenting cells in the presence of a human liver cell membrane preparation (HLM), was determined by 3H-thymidine incorporation. Frequency of LMSTL, calculated by Poisson distribution, ranged from 1/3375 to 1/65000 (median 1/20000) in aCAH, being 10 to 20 times higher than in controls (median 1/292961, range 1/30100-1/1286053, p<0.01). Children with aCAH have up to a twenty fold increase in the number of circulating T lymphocytes specifically directed to liver cell membrane antigens emphasising their possible involvement in immune mediated liver damage.
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Wen, L., Mowat, A., Mieli-Verctani, G. et al. FREQUENCY OF LIVER MEMBRANE SPECIFIC T LYMPHOCYTES IN AUTOIMMUNE CHRONIC ACTIVE HEPATITIS (aCAH). Pediatr Res 27, 530 (1990). https://doi.org/10.1203/00006450-199005000-00029
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DOI: https://doi.org/10.1203/00006450-199005000-00029