Abstract
Specific IGF-1 binding has been demonstrated on isolated human platelets. Maximal specific binding activity of J-125-recomb.-IGF-1 was measured after 8 h incubation at 10°C in 0.1 M Hepes-buffer pH 7.4 (cont. electrolytes). For 4×10(7) platelets maximum specific binding of J-125-IGF-1 was 1.6% with an unspec. binding of 0.3%. 1/2 max.binding was shown at 20 ng/ml IGF-1, the affinity constant was 0.67×10 (9)xM(−1). IGF-1 internalisation experiments were performed by preincubation with J-125-IGF −1 and dissociation of labeled hormone after cell placement into IGF-1 free medium or displacement by inactive IGF-1. Increasingly incomplete dissociation of J125-IGF-1 was demonstrated for preincubation times > 1h. These results are confirmed by diminished displacement of cell-bound J-125-IGF-1 by unlabeled hormone after a preincubation period of >1h. Preliminary results indicate that lysed platelets contain remarkable amounts of IGF-1. - It has to be considered that platelets serve as a reservoir from which IGF-1, following a local injury, is released after aggregation, and together with PDGF might stimulate fibroblast growth in wound healing.
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Hartmann, K., Loibl, R., Schmitt, A. et al. 107 INSULIN-LIKE GROWTH FACTOR-1 RECEPTOR INTERNALISATION INTO HUMAN PLATELETS. Pediatr Res 24, 535 (1988). https://doi.org/10.1203/00006450-198810000-00128
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DOI: https://doi.org/10.1203/00006450-198810000-00128