Abstract
Genomic DNA extracted from leukocytes of 18 French subjects of normal stature was analysed by Southern blot. Specific hybridization was done with the EcoRI-Bam HI insert of the h-IGF I cDNA probe (FEBS Lett., 1986, 196:108) (S. A. : 1.5-2. 10 cpm/μg) and washing done at 65°C (0.1x SSC, 0.1% SDS). DNA digested with Hind III yielded fragments of 7.7, 6.7, 5.1, 4.3 and 3.5 kb. The 4.3 kb fragment may have been a partial digestion product despite the high concentrations used (4U/ug, overnight). Six of the DNA samples had an additional 5.6 kb fragment showing Hind III RFLP. Another study with Pvu II yielded 7.3, 4.8, 3.7(partial digest?), 2.7 and 1,4 kb fragments and an additional polymorphic fragment of 5 kb. Polymorphic Pvu II and Hind III sites are linked; the heterozygous pattern appeared in 33% of the samples studied. Normal restriction patterns were seen in the DNA of 6 subjects with growth disorders (Laron's dwarfism, constitutionally short and tall children), where Hind III and Pvu II RFLPs were observed. Analysis with Hind III of 2 Pygmy DNA samples showed normal restriction patterns, without RFLP. No RFLP was found in the IGF I gene of normal French DNA samples when Bam HI (n=10; 17.6, 9.8, 7 kb, a minor 1 and 0.7 kb fragments), EcoRI (n=9; 7, 6.2, 4.1 and 1 kb) or Taq I (n=8; 10.4, 5, 3.6, 2.5 and 1.6 kb) were used. Taq I digestion of 2 Pygmy DNAs gave normal restriction patterns.
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Schneid, H., Le Bouc, Y., Noguiez, P. et al. 105 RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) OF THE INSULIN-LIKE GROWTH FACTOR 1 (1GF 1) GENE IN FRENCH SUBJECTS. Pediatr Res 24, 534 (1988). https://doi.org/10.1203/00006450-198810000-00126
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DOI: https://doi.org/10.1203/00006450-198810000-00126