Abstract
Integrated plasma concentrations of GH were studied in 14 childrer with constitutional growth delay using 7 different RIAs.
GH levels in 24h pool plasma samples measured in the same samples by the different assays varied widely(up ×3 the lowest value). The assay which uses monoclonal Abs (HYBRITECH) gave the lowest values; those using polyclonal Abs gave in general higher values(×2 the monoclonal assay)but they also varied widely among themselves(×2 between lowest and highest), hierarchy being : SERONO <DPC ≤SORIN < BEHRING <BIOMERIEUX ≤CIS .
The BIOMERIEUX(B) and SORIN(S) assays were applied to all 30' samples in addition to the pool 24h, day and night samples. The GH levels in 24h pool samples from the 14 children were(M∓SD):4.6∓2.7 ng/ml according to the B assay(11.4 to 1.2) and 2.9∓1.2 according to the S assay(5.7to0.8). The average ratio between the values given by the two assays(B/S)was 1.51∓0.32(2.0 to 1.0). A highly significant correlation was observed between the two assays(r=0.97)but the divergence was maximal at the highest levels of GH and a positive correlation between the B/S ratio and the GH concentrations has been documented(r=0.72, p<0.01). There is no correlation between B/S ratio and the maximum GH concentration attained(B/S=1.27 ∓ 0.18). The last is similar in GH response to Propanolol+Exercise. In conclusion, the results of the GH integrated concentrations are strongly influenced by the RIA used, probably because they measure different forms of the GH molecule.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Audí, L., Granada, M., Sanmartí, A. et al. 40 INTEGRATED CONCENTRATIONS OF GH IN CHILDREN WITH CONSTITUTIONAL GROWTH DELAY. RESULTS FROM SEVEN DIFFERENT RIAs. Pediatr Res 24, 523 (1988). https://doi.org/10.1203/00006450-198810000-00061
Issue Date:
DOI: https://doi.org/10.1203/00006450-198810000-00061