Abstract
Cultured bovine adrenal cells (BA) contained IGF type I receptors which have been characterized by cross-linking (125 KD) and affinity experiments (KD = 1.4 ± 0.3×10−9M). IGF-I had small mitogenic effects but potentiated the effect of fibroblast growth factor (FGF). Moreover, IGF-I enhanced in a time- and dose-dependent manner (ED50 ⋍ 10−9 M) the number of angiotensin-II (A-Il) receptors (2-3 fold), the cAMP response to ACTH (3 fold) and the steroidogenic response to both hormones (4-6 fold). Moreover, BA cells cultured in serum-free medium secreted, under basal conditions, an IGF-I-like material (5 ± 1.5 ng/106 cells/48 h, n = 7), the secretion of which was stimulated 8, 4.5 and 3 fold by ACTH, A-II and FGF respectively but not by growth hormone. The effect of FGF and those of ACTH or A-II were additive. Further characterization of the IGF-I-like material was achieved by affinity chromatography (using monoclonal anti-IGF-I antibodies) of conditioned medium from cells which were incubated for 48 h with labeled amino-acids.
The present results demonstrated that 1) BA cells contained IGF type I receptors and this peptide is required for the maintenance of BA differentiated function; 2) BA secreted an IGF-I like peptide, the secretion of which is regulated by the specific trophic hormone of these cells. Thus, IGF-I might play an autocrine role in the maintenance of differentiated adrenal functions and in their regulation by the specific trophic hormones.
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Penhoat, A., Chatelain, P., Jaillard, C. et al. 32 POTENTIAL AUTOCRINE ROLE OF IGF-I IN THE MAINTENNANCE OF DIFFERENTIATED ADRENAL FUNCTIONS. Pediatr Res 24, 522 (1988). https://doi.org/10.1203/00006450-198810000-00053
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DOI: https://doi.org/10.1203/00006450-198810000-00053