Abstract
S. typhimurium strains TML and W118 isolated from humans with cholera-like diarrhoea invaded rabbit ileum in vivo and induced fluid secretion. Avirulent strains, LT7, M206 and SL1027, invaded without inducing fluid secretion; Thaxl was non-invasive and non-fluid secreting. We have tested strains of S. typhimurium of varying virulence for ability to interact with rabbit ileal mucosa in an asymmetric organ culture system to determine if differences in the degree of mucosal association could explain differences in virulence. Strains were also tested for motility and presence of flagellae. Mean(SEM) numbers of organisms (log cfu/g wet wght ileal mucosa) associated with the mucosa after 30min incubation and washing were: TML 6.17(0.17;n=15) W118 6.14(0.27;n=7) LT7 6.31(0.11;n=5) M206 6.14(0.11;n=5) SL1027 5.49(0.21;n=6 P vs TML<0.025) Thaxl 4.88 (0.15;n=7 P vs TML<0.0005). All strains except Thaxl had flagellae, and all except Thaxl and SL1027 were motile. Thus flagellae may enhance association of strains of S. typhimurium with ileal mucosa. When strains are motile association is further enhanced. Decreased association with ileal mucosa may explain in part the decreased virulence of strains SL1027 and Thaxl, but not LT7 and M206. Thus factors other than functional adhesins are involved in determining virulence of S. typhimurium.
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Candy, D., Worton, K., Wallis, T. et al. 61 INTERACTION OF STRAINS OF Salmonella typhimurium OF DIFFERENT VIRULENCE WITH ILEAL MUCOSAE in vitro. Pediatr Res 24, 415 (1988). https://doi.org/10.1203/00006450-198809000-00084
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DOI: https://doi.org/10.1203/00006450-198809000-00084