Abstract
The relative effect of glucose or lipids on whole body protein metabolism was investigated by a randomised crossover study in 5 infants (age 3-8 months) steadily growing on parenteral feeding, with constant protein (2,8 + 0,2 g/kg.d) and energy (103 + 3 kcal/kg.d) intakes. Energy was provided as glucose and as an isocaloric glucose lipid mixture, with 50 mg/kg.d L carnitine. Net glucose and fat oxidation rates were measured by indirect calorimetry (IC) for 6 hours. After 120 mn, a primed-constant (Ll13C) leucine infusion was started. Breath CO2 and plasma samples were obtained at 0, 60, 120 and every 30 mn. Enrichments of plasma ketoisocaproate and leucine were measured by mass spectrometry (MS) and 13CO2 production rate by using IC and MS data at steady state enrichment. Lipogenesis (2 + 0,8 g/kg.d) was present with glucose, whole body protein synthesis (PS) and breakdown (PB) rates amounting to 8,3 + 0,6 and 8,1 + 0,9 g/kg.d, respectively resulting in a 0,2 + 0,7 g/kg.d net synthesis (PNS) rate; with the glucose-lipid mixture, fat oxidation rate was 3,8 + 0,6 g/kg.d (p<0,001), PS rate 7,4 + 0,5 but PB was only 5,5 + 0,8 g/kg.d (p<0,01), resulting in a 1,9 g/kg.d PNS. Thus, the glucose-lipid mixture resulted both in significantly improved whole body protein accretion and reduced fat deposition rates, when compared to higher glucose infusion rates.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bresson, J., Bader, B., Mariotti, A. et al. 36 ENERGY-SOURCE RELATED DIFFERENCES IN WHOLE BODY PROTEIN METABOLISM MEASURED IN PARENTERALLY FED INFANTS, BY COMBINED STABLE ISOTOPIC LABELLING AND INDIRECT CALORIMETRY. Pediatr Res 24, 411 (1988). https://doi.org/10.1203/00006450-198809000-00059
Issue Date:
DOI: https://doi.org/10.1203/00006450-198809000-00059