Abstract
To determine the clearance of endotracheally instilled artificial porcine derived surfactant (APS) from the lung, premature surfactant deficient rabbits were sacrificed at 30, 60, and 120 min. following surfactant instillation. APS was determined in lung tissue specimens using a monoclonal antibody against APS apoprotein a peroxidase staining technique and a counting grid. In 18 rabbits APS was instilled endotracheally, 6 did not receive surfactant (controls). Results: The number of surfactant positive points in alveoli was 38.2±20.0, 13.8±12.0 and 3.3±4.4 at 30, 60, and 120 min. after instillation resp. (p<0.05). In the perivascular spaces we found 0, 4.0±0.1 and 14.5±7.3 resp. (p<0.05). No surfactant positive points were found in controls. We conclude that endotracheally instilled APS is cleared from the lungs of surfactant deficient premature rabbits within 2 hours. Presumably via the lymphatic ducts in the perivascular spaces.
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Oetomo, S., Schoots, C., Lachmann, B. et al. 108: CLEARANCE OF ENDOTRACHEALLY INSTILLED SURFACTANT IN PREMATURE SURFACTANT DEFICIENT RABBITS. Pediatr Res 24, 278 (1988). https://doi.org/10.1203/00006450-198808000-00133
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DOI: https://doi.org/10.1203/00006450-198808000-00133