Abstract
The role of NK and T cells for rejection of fully allogenic (BALB/c into C57B1/6J) T cell depleted BM was investigated in a murine model using congenitally T (nu/nu) or NK (bg/bg) deficient mutants as recipients. All recipient animals were conditioned with 9.5 Gy TBI.
Results: 1) Injection of 1×107 T-undepleted BM resulted in engraftment in more than 80% of the C57 recipients. Normal C57 and both deficient mutants accepted the T-undepleted grafts equally well. 2) Injection of 1×107 T-depleted BM (1% contaminating T cells) led to an over all graft failure rate of 60%. 3) Whereas graft failure was observed in approximately 80% of normal C57 and 80% of nu/nu, only 33% of C57 bg/bg rejected the T-depleted graft.
Discussion: Transplantation of T-depleted BM is known to result in an increased rate of graft failures. Surprisingly, the incidence of graft failures was the same in normal (80%) and T deficient (80%) recipients. A rejection rate of 80% in the nu/nu mutant and the fact that no late graft failure occured argues against a significant role of classical T cell mediated rejection in this experimental model. The results clearly show that NK deficient animals had the lowest rate of graft failures. Results will be discussed in light of Hh antigens and NK target cell structures.
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Schofer, O., Büttner, H., Zepp, F. et al. 85 ROLE OF NK CELLS IN REJECTION OF T-DEPLETED BM GRAFTS. Pediatr Res 24, 275 (1988). https://doi.org/10.1203/00006450-198808000-00111
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DOI: https://doi.org/10.1203/00006450-198808000-00111