Abstract
Can oleate and BOHB substitute for glucose as cerebral energy fuels after hypoxia? Oleate-U-14C or BOHB-3-14C were added to 7 days old cultures after 4 hours preincubation at 0 or 4 mM glue under normoxia or hypoxia. During 3 hours of incubation of all cultures under normoxia, 14CO2 was collected. Defining CO2-production at 4 mM glue as 100%, CO2-production at 0 vs. 4 mM gluc was 156±40% in normoxia and 162±24% after hypoxia for BOHB, the corresponding values for CO2-prcduction from oleate were 218±66 and 261±50% (mean± 1 SD, n=15-25, p 0 vs 4 mM gluc<0.001). CO2-production after hypoxia was compared with CO2-production in normoxia both at 0 and 4 mM glue (100%=CO2-production under normoxia). After hypoxia, BOHB was significantly less converted to 002:86±28% at 0 (n=15-20, p< 0.005), 86±38% at 4 mM glue (n=19-21, p< 0.01). Oleate was also less converted to CO2 after hypoxia at 4 mM glue (87± 34%, n=23-25, p < 0.01). At 0 gluc, however, CO2-production from oleate was higher after hypoxia than in normoxia (112±46%, n= 39-41, p< 0.005). In our system, oleate and BOHB arc alternate cerebral energy fuels for glucose in normoxia and hypoxia.
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Bossi, E., Herschkcwltz, N. & Kohler, E. 67 UTILIZATION OF OLEATE AND D-BETA-OH-BUTYRATE (BOHB) AS ALTERNATE ENERGY FUELS IN BRAIN CELL CULTURES OF NEWBORN MICE AFTER HYPOXIA AT DIFFERENT GLUCOSE CONCENTRATIONS. Pediatr Res 24, 272 (1988). https://doi.org/10.1203/00006450-198808000-00093
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DOI: https://doi.org/10.1203/00006450-198808000-00093