Abstract
ABSTRACT: We immunized 117 children with either Haemophilus influenzae type b polysaccharide vaccine or type b polysaccharide coupled to an outer membrane protein of group B Neisseria meningitidis (conjugate vaccine), and measured the IgG, IgGl, and IgG2 subclass composition of antibody to type b polysaccharide in postimmunization sera by ELISA. The IgG responses of 51 children, 24–83 months of age, immunized for the first time with the conventional type b vaccine consisted of both IgGl and IgG2 antibody (respective geometric means of 2.24 and 0.77 μg/ml). In contrast, the IgG responses of 28 infants, 2–17 months of age, immunized with conjugate vaccine were predominantly or exclusively IgGl (geometric mean IgGl and IgG2 antibody concentrations of 1.92 and 0.19 μg/ml). A total of 38 children was primed with conjugate vaccine between 2 and 17 months of age and boosted approximately 1 yr later. The 28 children boosted with type b polysaccharide vaccine showed memory antibody responses consisting of both IgGl and IgG2 (respective geometric means of 12.7 and 4.8 μg/ml); the 10 children boosted with conjugate vaccine showed a similar pattern of IgG subclass responses (respective geometric means of 20.8 and 5.1 μg/ml, p > 0.4 compared to the respective geometric mean IgGl and IgG2 values of the group boosted with polysaccharide). Thus, in children 24–83 months of age, immunization with conventional type b polysaccharide vaccine generally elicits both IgGl and IgG2 responses, with a slight predominance of IgGl. In contrast, in infants 2–17 months of age, immunization with conjugate vaccine evokes a restricted IgGl antibody response to the polysaccharide but primes for both IgGl and IgG2 responses to a booster immunization with conventional polysaccharide or conjugate vaccine.
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Granoff, D., Weinberg, G. & Shackelford, P. IgG Subclass Response to Immunization with Haemophilus influenzas Type b Polysaccharide-Outer Membrane Protein Conjugate Vaccine1. Pediatr Res 24, 180–185 (1988). https://doi.org/10.1203/00006450-198808000-00008
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DOI: https://doi.org/10.1203/00006450-198808000-00008
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