Abstract
Human peripheral blood T cells were stimulated to proliferate when cultured with subraitogenic doses of phorbol myristate acetate (PMA) and goat antibodies to ecto-5′-nucleotidase (ecto-5′-NT). The degree of proliferation, as measured by 3H-thymidine incorporation on day 3, was similar to that achieved by stimulation with phytohemagglutinin (PHA). Anti-5′-NT antibodies had no effect on PHA-induced proliferation. Maximal stimulation was achieved with 0.6-1.0 ng PMA/ml and 150 μg/ml of IgG isolated from a goat anti-5′-NT antiserum. Both intact IgG and F(ab')2 fragments were stimulatory. IL-2 receptor expression and IL-2 secretion were also induced by anti-5′-NT antibodies and PMA. As expected, anti-5′-NT antibodies and PMA did not induce the proliferation of ecto-5′-NT− T cells isolated by cell sorting. Pretreatment of total T cells with phosphatidylinositol (PI)-specific phospholipase C removed 80% of the ecto-5′-NT activity from the cell surface and also inhibited the ability of the cells to proliferate in response to anti-5′-NT antibodies and PMA by 84%. Thus, the activation signal provided by anti-ecto-5′-NT antibodies is apparently transduced by a form of the enzyme which is attached to the membrane via PI-linkage. These data suggest that ecto-5′-NT may play a role in lymphocyte activation as has been proposed for other PI-linked lymphocyte surface proteins including Thy-l, T cell activating protein, TAP, and the rat alloantigen RT-6.
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Thompson, L., Ruedi, J. & Low, M. 161 ANTI-5′-HUCLEOTIDASE ANTIBODIES CAUSE HUMAN PERIPHERAL BLOOD T CELLS TO PROLIFERATE. Pediatr Res 24, 138 (1988). https://doi.org/10.1203/00006450-198807000-00185
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DOI: https://doi.org/10.1203/00006450-198807000-00185