Abstract
Radiolabeled adenine (1-20 μM) is taken up and accumulated intracellularly above equilibrium levels by human erythrocytes suspended in phosphate-free isotonic media at 37°C. Adenine, adenosine, and adenine nucleotides are the chief labeled compounds present in the cells under these conditions. The incorporation of labeled adenine is strongly inhibited by cold adenine (5-50 μM), but not by hypoxanthine (up to 2 mM). The rate of adenine incorporation is independent of the oxygen partial tension and reaches a maximum value around physiological pH. The rate of incorporation of the purine base increases, if the cells are preincubated for 10-30 min at 37°C in the presence of 40-100 mU/ml xanthine oxidase. The same result can be obtained, using erythrocytes enriched with PRPP or with 14-C IMP. In the latter case, adenine uptake is associated with the release of equi-molar amounts of hypoxanthine which results from the catabolism of intracellular IMP.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Salerno, C., Werner, A., Siems, W. et al. 134 ADENINE UPTAKE BY HUMAN ERYTHROCYTES. Pediatr Res 24, 133 (1988). https://doi.org/10.1203/00006450-198807000-00158
Issue Date:
DOI: https://doi.org/10.1203/00006450-198807000-00158