Abstract
In phosphate-free isotonic media, 14-C hypoxanthine is taken up by human erythrocytes but less than 5% of the purine base is metabolized to IMP. The kinetics of uptake is strongly dependent on temperature and on initial hypoxanthine concentration in the suspending medium. At 0-5°C, but not at higher temperatures, the addition of cold hypoxanthine to the incubation mixture leads to a decrease of the amount of labeled base taken up by the cells. Experiments performed at equilibrium show that the total amount of radioactive material taken up by erythrocytes is a hyperbolic function of the total (labeled plus cold) hypoxanthine concentration. A decrease in the rate of 14-C hypoxanthine uptake is observed when the cells are preincubated in the presence of proteinase K. By contrast, when the uptake is studied at 20-37°C, the time course of hypoxanthine uptake by protease-treated cells appears to be very similar to that observed using native cells. At these temperatures, hypoxanthine is taken up by the cells until its intracellular concentration becomes approximately equal to the extracellular one.
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Salerno, C., Capuozzo, E., Giacomello, A. et al. 133 INFLUENCE OF THE TEMPERATURE ON HYPOXANTHINE TRANSPORT THROUGH HUMAN ERYTHROCYTE MEMBRANES. Pediatr Res 24, 133 (1988). https://doi.org/10.1203/00006450-198807000-00157
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DOI: https://doi.org/10.1203/00006450-198807000-00157