Abstract
The conversion of hypoxanthine and xanthine to urate via xanthine oxidase (Xanthine: oxygen oxidoreductase (E.C. 1.1.3.22.) may when ischaemic tissues are re-oxygenated generates radical oxygen species. Such ischaemia may be involved in the adverse effects of non-steroidal anti-inflammatory drugs (NSAIDS) on the human intestine where high levels of xanthine oxidase are known to be present. However little qualitative or quantitative information concerning the nucleotide content of intestine is available.
We have determined the nucleotide profiles in freeze-clamped sections of rat intestine (stomach, jejunum, ileum and colon) and for comparison liver and heart by anion-exchange HPLC and quantified the individual nucleotides. The mean of 10 analyses are summarised below
The results suggests that the intestine is a major site of nucleotide metabolism with nucleotide concentrations equivalent to liver rather than heart. A slight concentration gradient down the gut was observed. During short-term absorption studies using saline, glucose, acetate and citrate neither energy charge nor absolute levels of nucleotides changed significantly. Rapid degradation of nucleotides was induced by warm ischaemia and NSAID's.
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Perrett, D., Watson, A. & Rolston, D. 111 QUALITATIVE AND QUANTITATIVE STUDIES ON THE NUCLEOTIDES OF INTESTINAL MUCOSA. Pediatr Res 24, 129 (1988). https://doi.org/10.1203/00006450-198807000-00135
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DOI: https://doi.org/10.1203/00006450-198807000-00135