Abstract
Purine 5′-nucleotidase, formerly termed cytosol 5′-nucleotidase (Tsushima, K. (1986) Adv Enzyme Regul 25, 181), is one of soluble nucleotidases including pyrimidine 5′-nucleotidase and deoxyribo-nucleotidase, and deferentially hydrolyzes IMP, GMP, and AMP in the presence of Mg++. The enzyme has been investigated to have allosteric properties characterized by activation by ATP, ADP, 2, 3-diphosphoglycerate, and diadenosine tetraphosphate. Purine 5′-nucleotidase, has been purified from various sources, but structural studies seem to be incomplete. The subunit molecular mass of chicken liver enzyme, which was earlier reported to be 51 kDa upon SDS-PAGE, was reinvestigated. By immunoblot analyses after SDS-PAGE, a crude fraction from the liver homogenized in the presence of leupeptin showed multiple bands around 57 kDa, and SDS-extracted proteins directly from the liver exhibited a single immunoreactive 70-kDa band. In vitro translation products using chicken liver polysomes also showed a radioactive 70-kDa band after immunoprecipitation. Immunocytocheraical study showed that the antigen was exclusively located in the cytoplasmic matrix of chicken liver sinusoidal and parenchymal cells, suggesting that physiological processing might not be the case with chicken liver enzyme. These results indicate that the subunit molecular mass of chicken liver purine 5′-nucleotidase might be 70 kDa, and the enzyme is the first case to be morphologically located in the cytosol among several known cytosolic nucleotidases.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oka, J., Ozasa, H., Itoh, R. et al. 103 PURINE 5′-NUCLEOTIDASE—ITS REESTIMATED SUBUNIT MOLECULAR MASS AND IMMUNOCYTOCHEMICAL LOCALIZATION IN CHICKEN LIVER. Pediatr Res 24, 128 (1988). https://doi.org/10.1203/00006450-198807000-00127
Issue Date:
DOI: https://doi.org/10.1203/00006450-198807000-00127