Abstract
In order to investigate the ability of GH and GH-releasing hormone (GH-RH) to modulate LP “in vitro”, peripheral blood lymphocytes (PBL) from healthy adults were stimulated with PHA in the presence of increasing, concentrations of biosynthetic GH (Somatonorm) (0.425×103−501.5×103 ng/ml) and of GH-RH (1-29, Kabi-Vitrum) (0.006 -50 ug/ml). The results obtained show that low doses of GH and GH-RH increase LP “in vitro”, while high concentrations of the hormones (GH:8.5×103 ng/ml; GH-RH:0.048 μg/ml) induce a progressive decrease of PHA-induced LP. No differences in cell viability were observed with and without GH and GH-RH and comparable percentages of TAC+ cells and of IL-2 production were found in all conditions tested. Nevertheless, when exogenous recombinant IL-2 was added to PHA-induced PBL culture with high doses of GH or GH-RH, a reconstitution of LP was observed. Further experiments seem to exclude the presence of T-suppressor lymphocyte induction by GH and GH-RH. The present results suggest the hypothesis that high doses of GH and GH-RH may interfere with the binding between IL-2 and its membrane receptor on activated lymphocytes.
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Bozzola, M., Moretta, A., Valtorta, A. et al. MODULATING EFFECT OF GROWTH HORMONE (GH) ON “IN VITRO” LYMPHOPROLIFERATION (LP). Pediatr Res 23, 118 (1988). https://doi.org/10.1203/00006450-198801000-00102
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DOI: https://doi.org/10.1203/00006450-198801000-00102