Abstract
LH and FSH values were studied in 30 healthy boys (CA:11.3-14.6 yrs, BA:10.3-16.2 yrs (TW2), Tanner stage G1-G5). Informed consent was obtained. Blood was sampled at 10′ intervals from 12.00-18.00h and from 24.00-06.00h. An IRMA employing 2 monoclonal antibodies with a sensitivity of LH and FSH of 0.10 IU/l and 0.25 IU/l respectively was used. Testosterone (T) was measured every hour applying a RIA with a sensitivity of 1.0 nmol/1. Results of mean FSH, of mean LH, of mean LH pulse amplitude (pA), of LH pulse number (pN), of mean Nadir Interval in min.(NI) and of mean T are presented as the median per Tanner stage.
Conclusion: At the onset of puberty(Gl) LH is undetectable during the day, while during the night LH is secreted in a pulsatile pattern. During puberty LH level, pA and pN increase, the night ahead. In C5 pN decreases, while pA stil 1 increases, presumable as a result of an increased negative feedback action of T. In contrast to LH, diurnal FSH is detectable in Gl, shows a clear increase already in G2 and has no sleep-wake pattern.
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Wennink, J., Delemarre-van de Waal, H. 132 DIURNAL AND NOCTURNAL LH AND FSH SECRETION IN BOYS THROUGHOUT PUBERTY ESTABLISHED WITH A SENSITIVE IRMA. Pediatr Res 24, 539 (1988). https://doi.org/10.1203/00006450-198810000-00153
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DOI: https://doi.org/10.1203/00006450-198810000-00153