Abstract
A 16 month-old girl was seen for precocious puberty (breast, pubic hair). Plasma levels (ng/dl) of progesterone (P) (574-1830), 17OH-progesterone (OHP) (181-195) and estradiol (E2) (23-40) were in the range of an adult luteal phase. Androgens were moderately elevated. LH/FSH were suppressed. Dispersed cells were prepared from either luteinized (pool A) or interstitial (pool B) tumoral tissue. The secretory pattern was established after 1 day of culture and was similar to that in the cystic fluid of the tumor. Viability of the culture was checked by the maintenance of the steroid secretory pattern the next 3 days. On day 9th, the cellswere stimulated by either hCG (10−9 M), FSH (1 ng), forskolin (FK) 10−5 M. cAMP (pmol) was measured after 1 h, OHP and P (ng) and E2 (pg) at 24 h. Results are given in the Table as mean production per 10−6 cells (*p < 0.05). Also, hCG receptors (sites/cell) were found but low in A (2800) and B (1400); FSH receptors were found only in A (940) on day I and were comparable on day 9.
These studies show that the tumoral cells have a high autonomous steroidogenic activity, which was poorly stimulated by gonadotropins due to the low levels of receptors. However the adenylate cyclase and the steroidogenesis remained very sensitive to FK alone or associated with gonadotropin.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Forest, M., Saez, J. & David, M. 124 IN VIVO AND IN VITRO STUDIES OF A JUVENILE GRANULOSA CELL TUMOR. Pediatr Res 24, 537 (1988). https://doi.org/10.1203/00006450-198810000-00145
Issue Date:
DOI: https://doi.org/10.1203/00006450-198810000-00145